College of Food Science, Woosuk University , Samrea-Up, Wanju-Kun, Jeonbuk Province 565-701, Republic of Korea.
J Agric Food Chem. 2012 Oct 10;60(40):10015-26. doi: 10.1021/jf302944p. Epub 2012 Oct 1.
Liquid chromatography-time-of-flight mass spectrometry (LC-TOF/MS) was established for the simultaneous separation, identification, and quantification of gingerol-related compounds in ginger products. The established method has been shown to provide a satisfactory linearity (r > 0.999) in a wide range (5-5000 ng/mL), low limits of detection and quantification, high precision, and inter- and intraday repeatability. The detection sensitivity of gingerols and shogaols by TOF/MS was 70-100 times higher than conventional UV detection at 288 nm. In this study, 19 ginerol-related compounds in the samples were identified and quantified by the established LC-TOF/MS method. The dried ginger powder products contained the highest quantity of gingerol-related compounds (7126.3-13789.0 μg/g), followed by fresh ginger products (2007.9-2790.0 μg/g), powdered ginger tea products (77.29-81.75 μg/g), and hot water ginger extracts (54.59-123.23 μg/mL). Shogaols were not found in fresh gingers. This paper represents the first report on the LC-TOF/MS analysis for the simultaneous characterization and quantification of gingerol-related compounds in ginger products.
建立了液质联用飞行时间质谱（LC-TOF/MS）法，用于同时分离、鉴定和定量姜产品中的姜辣素类化合物。该方法在较宽的浓度范围内（5-5000ng/mL）表现出令人满意的线性（r＞0.999）、较低的检测限和定量限、较高的精密度和日间、日内重复性。与传统的 288nm UV 检测相比，TOF/MS 对姜辣素和姜烯酚的检测灵敏度提高了 70-100 倍。本研究采用建立的 LC-TOF/MS 方法对样品中的 19 种姜辣素类化合物进行了鉴定和定量分析。干姜粉产品中姜辣素类化合物含量最高（7126.3-13789.0μg/g），其次是鲜姜产品（2007.9-2790.0μg/g）、姜粉茶产品（77.29-81.75μg/g）和热水姜提取物（54.59-123.23μg/mL）。鲜姜中未发现姜烯酚。本文首次报道了采用 LC-TOF/MS 法同时对姜产品中姜辣素类化合物进行特征化和定量分析。
