Bentley P J, Grubb B R
Department of Anatomy, Physiological Sciences and Radiology, North Carolina State University, College of Veterinary Medicine, Raleigh 27606.
Invest Ophthalmol Vis Sci. 1990 Jan;31(1):153-5.
A procedure is described, using a freezing microtome and measurements of tissue slice volume, to obtain serial sections of the lens for analysis of components from one side of the organ to the other. The concentrations of zinc (analyzed by atomic absorption spectrophotometry) were similar in serial sections taken across the lens from the anterior to the posterior surface. The levels of the zinc metalloenzyme superoxide dismutase were, however, much higher in the anterior sections. This quite simple procedure may be useful for analyzing the distribution of lenticular components.
本文描述了一种使用冷冻切片机和测量组织切片体积的方法，以获取晶状体的连续切片，用于分析从器官一侧到另一侧的成分。通过原子吸收分光光度法分析的锌浓度，在从晶状体前表面到后表面的连续切片中相似。然而，锌金属酶超氧化物歧化酶的水平在前部切片中要高得多。这种非常简单的方法可能有助于分析晶状体成分的分布。
