Section of Infectious Diseases, Department of Medicine, Baylor College of Medicine, Houston, TX, USA.
J Appl Microbiol. 2012 Dec;113(6):1530-9. doi: 10.1111/j.1365-2672.2012.05432.x. Epub 2012 Sep 17.
We hypothesized that pretreating urinary catheters with benign Escherichia coli HU2117 plus an antipseudomonal bacteriophage (ΦE2005-A) would prevent Pseudomonas aeruginosa biofilm formation on catheters--a pivotal event in the pathogenesis of catheter-associated urinary tract infection (CAUTI).
Silicone catheter segments were exposed to one of four pretreatments (sterile media; E. coli alone; phage alone; E. coli plus phage), inoculated with P. aeruginosa and then incubated up to 72 h in human urine before rinsing and sonicating to recover adherent bacteria. Pseudomonas aeruginosa adherence to catheters was almost 4 log(10) units lower when pretreated with E. coli plus phage compared to no pretreatment (P < 0.001) in 24-h experiments and more than 3 log(10) units lower in 72-h experiments (P < 0.05). Neither E. coli nor phage alone generated significant decreases.
The combination of phages with a pre-established biofilm of E. coli HU2117 was synergistic in preventing catheter colonization by P. aeruginosa.
We describe a synergistic protection against colonization of urinary catheters by a common uropathogen. Escherichia coli-coated catheters are in clinical trials; adding phage may offer additional benefit.
我们假设用良性大肠杆菌 HU2117 预处理导尿管，再加上抗假单胞菌噬菌体（ΦE2005-A），可以防止铜绿假单胞菌在导尿管上形成生物膜，这是导管相关性尿路感染（CAUTI）发病机制中的关键事件。
硅胶导管段接受四种预处理之一（无菌介质；单独的大肠杆菌；单独的噬菌体；大肠杆菌加噬菌体），用铜绿假单胞菌接种，然后在人尿中孵育长达 72 小时，然后冲洗和超声以回收附着的细菌。与无预处理相比，用大肠杆菌加噬菌体预处理的铜绿假单胞菌对导管的粘附几乎低 4 个对数（10）单位（P < 0.001），在 72 小时实验中低 3 个对数（10）单位以上（P < 0.05）。单独的大肠杆菌或噬菌体都没有产生显著的减少。
噬菌体与预先建立的大肠杆菌 HU2117 生物膜的组合在预防铜绿假单胞菌对导管的定植方面具有协同作用。
我们描述了一种针对常见尿路病原体定植导尿管的协同保护作用。涂有大肠杆菌的导尿管正在临床试验中；添加噬菌体可能会带来额外的好处。
