[过氧化物酶体增殖物激活受体1、过氧化物酶体增殖物激活受体6和胶质纤维酸性蛋白在人脑星形细胞瘤中的表达及其临床意义]
[Expressions of peroxiredoxin 1, peroxiredoxin 6 and GFAP in human brain astrocytoma and their clinical significance].
作者信息
Zhou Jinqiao, Liu Qiuhong, Wang Jingtao, Guo Xinbin, Song Laijun
机构信息
First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2012 Sep;32(9):1255-9.
OBJECTIVE
To characterize the expressions of peroxiredoxin 1 (Prx1), peroxiredoxin 6 (Prx6) and glial fibrillary acidic protein (GFAP) in human brain astrocytoma and explore their clinical significance.
METHODS
The protein and mRNA expression levels of Prx1, Prx6 and GFAP in human brain astrocytoma and normal brain tissue specimens were determined by Western blotting, RT-PCR and immunohistochemistry.
RESULTS
The protein and mRNA expressions of Prx1 and Prx6 increased significantly in the order of normal brain tissue, grade II astrocytoma, grade III astrocytoma and grade IV astrocytoma (P<0.05). The protein and mRNA expressions of GFAP decreased significantly in grade III and IV astrocytoma compared with those in grade II astrocytoma and normal brain tissues (P<0.05).
CONCLUSION
Prx1 and Prx6 may play important roles in the invasion and malignant development of human brain astrocytoma, and may serve as biomarkers for evaluating the invasiveness, malignancy and prognosis of the tumor as well as potential molecular targets in astrocytoma therapy.
目的
探讨过氧化物还原酶1（Prx1）、过氧化物还原酶6（Prx6）和胶质纤维酸性蛋白（GFAP）在人脑星形细胞瘤中的表达特征，并探讨其临床意义。
方法
采用蛋白质免疫印迹法、逆转录-聚合酶链反应（RT-PCR）和免疫组织化学法检测人脑星形细胞瘤及正常脑组织标本中Prx1、Prx6和GFAP的蛋白及mRNA表达水平。
结果
Prx1和Prx6的蛋白及mRNA表达水平按正常脑组织、Ⅱ级星形细胞瘤、Ⅲ级星形细胞瘤和Ⅳ级星形细胞瘤的顺序显著升高（P<0.05）。与Ⅱ级星形细胞瘤和正常脑组织相比，Ⅲ级和Ⅳ级星形细胞瘤中GFAP的蛋白及mRNA表达水平显著降低（P<0.05）。
结论
Prx1和Prx6可能在人脑星形细胞瘤的侵袭和恶性进展中起重要作用，可作为评估肿瘤侵袭性、恶性程度和预后的生物标志物，以及星形细胞瘤治疗的潜在分子靶点。
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