Gong Daoyuan, Li Ziping, Zhuang Xiwei, Zhang Xiaolin, Kang Mei, Liao Yiping
Medical College of Foshan University of Science and Technology, Foshan 528000, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2012 Sep;32(9):1355-7.
To investigate the changes in seminal paraoxonase-1 (PON-1) activity in infertile male patients and assess the clinical value of seminal PON-1 examination in the diagnosis of male infertility.
Seminal PON-1 activity was detected by spectrophotometric method in the semen samples from 270 infertile male patients and 50 health fertile males (control), and the semen parameters were analyzed using a computer-assisted semen analysis system.
In the male infertility group, seminal PON-1 activity was 1.22∓0.76 U/L in the patients with normal semen parameters and 0.64∓0.54 in the patients with abnormal semen parameters, both significantly lower than that of the control group (3.17∓0.89 U/L, P<0.01). In patients with asthenospermia, the declined sperm motility was associated with decreased seminal PON-1 activity, which showed significant differences between patients with mild, moderate, and severe asthenospermia. Seminal PON-1 activity was positively correlated with the percentage of sperm viability (P<0.01), but inversely with the percentage of morphologically abnormal sperm (P<0.01). According to ROC curves, the area of seminal PON-1 activity under the curve was 0.907, showing a statistical significance (P<0.01).
The detection of seminal PON-1 activity can provide a laboratory evidence for the diagnosis of male infertility.
探讨男性不育患者精液对氧磷酶-1（PON-1）活性的变化，评估精液PON-1检测在男性不育诊断中的临床价值。
采用分光光度法检测270例男性不育患者及50例健康有生育能力男性（对照组）精液样本中的PON-1活性，并用计算机辅助精液分析系统分析精液参数。
男性不育组中，精液参数正常患者的精液PON-1活性为1.22±0.76 U/L，精液参数异常患者为0.64±0.54 U/L，均显著低于对照组（3.17±0.89 U/L，P<0.01）。在弱精子症患者中，精子活力下降与精液PON-1活性降低有关，轻度、中度和重度弱精子症患者之间存在显著差异。精液PON-1活性与精子存活率百分比呈正相关（P<0.01），与形态异常精子百分比呈负相关（P<0.01）。根据ROC曲线，精液PON-1活性曲线下面积为0.907，具有统计学意义（P<0.01）。
精液PON-1活性检测可为男性不育的诊断提供实验室依据。
