Janati Sima, Behmanesh Mohammad Amin, Biabani Ehsan, Poormoosavi Seyedeh Mahsa
Department of Obstetrics and Gynecology, School of Medicine, Research and Clinical Center for Infertility, Dezful University of Medical Sciences, Dezful, Iran.
Department of Histology, School of Medicine, Dezful University of Medical Sciences, Dezful, Iran.
J Reprod Infertil. 2025 Jan-Mar;26(1):13-18. doi: 10.18502/jri.v26i1.18777.
PON3 is associated with high-density lipoprotein (HDL) and plays a protective role against oxidative damage to low-density lipoprotein (LDL). The purpose of the current study was to investigate the effect of semen paraoxonase-3 (PON3) concentration on sperm parameters and DNA fragmentation in both fresh or post-thaw semen.
This study analyzed PON3 levels in 30 normospermic semen samples obtained from men who attended Dezful Infertility Center and provided informed consent. The samples were analyzed before and after cryopreservation for sperm motility, DNA fragmentation, and seminal PON3 levels. Data analysis was performed using SPSS software and statistical differences were evaluated using ANOVA. A p≤0.05 was considered statistically significant.
There was no significant difference in sperm morphology (p=0.37) and count (p=0.25) before and after freezing at different levels of PON3. The highest levels of progressive motility were observed in samples with the highest PON3 concentrations both before (p=0.01) and after freezing (p=0.02), whereas non-progressive motility was significantly greater in samples with the lowest PON3 concentrations at both time points (p=0.01). Sperm DNA fragmentation significantly decreased before or after freezing as PON3 levels increased (p=0.03).
This study suggests that PON3 levels may serve as an indicator for both sperm motility and DNA fragmentation pre- or post-cryopreservation, potentially contributing to future clinical research.
对氧磷酶3（PON3）与高密度脂蛋白（HDL）相关，对低密度脂蛋白（LDL）的氧化损伤起保护作用。本研究的目的是调查精液对氧磷酶3（PON3）浓度对新鲜或解冻后精液中精子参数和DNA片段化的影响。
本研究分析了从德兹富尔不孕不育中心招募的30名精子正常的男性精液样本中的PON3水平，这些男性均提供了知情同意书。对样本进行冷冻保存前后的精子活力、DNA片段化和精液PON3水平分析。使用SPSS软件进行数据分析，并采用方差分析评估统计学差异。p≤0.05被认为具有统计学意义。
在不同PON3水平下，冷冻前后精子形态（p=0.37）和数量（p=0.25）无显著差异。在冷冻前（p=0.01）和冷冻后（p=0.０2），PON3浓度最高的样本中观察到最高水平的前向运动，而在两个时间点PON3浓度最低的样本中，非前向运动显著更高（p=0.01）。随着PON3水平升高，冷冻前后精子DNA片段化显著降低（p=0.03）。
本研究表明，PON3水平可能作为冷冻保存前后精子活力和DNA片段化的指标，可能有助于未来的临床研究。
