Verit Fatma Ferda, Verit Ayhan, Ciftci Halil, Erel Ozcan, Celik Hakim
Department of Obstetrics and Gynecology, Harran University, Faculty of Medicine, Sanliurfa, Turkey.
J Androl. 2009 Mar-Apr;30(2):183-9. doi: 10.2164/jandrol.108.004929. Epub 2008 Oct 16.
Oxidative stress has been implicated in the pathogenesis of male infertility. Paraoxonase-1 (PON-1) is a high-density lipoprotein-associated antioxidant enzyme that prevents oxidative modification of low-density lipoprotein. Our aims in the study were to investigate 1) seminal PON-1 activity in subfertile men and 2) whether seminal PON-1 activity had any relationship to semen parameters. The study included 28 men with idiopathic subfertility, 32 subfertile men with abnormal semen parameters, and 30 fertile male volunteers. Seminal PON-1 activity was measured spectrophotometrically. Seminal total antioxidant status (TAS) and total oxidant status (TOS) were determined by using colorimetric methods. Oxidative stress index (OSI) was calculated as ([TOS/TAS] x 100). TOS and OSI were significantly higher and PON-1 activity and TAS were significantly lower in subfertile men with abnormal semen parameters than in men with idiopathic subfertility and fertile donors. PON-1 activity was also strongly correlated with sperm concentration (r = .68, P < .0001), motility (r = .58, P < .0001), and morphology (r = .62, P < .0001) in the overall group. The receiver operating characteristic curve analysis revealed a high diagnostic value for PON-1 activity with respect to male-factor subfertility, with an area under curve of .95 (95% confidence interval = 0.89-1.01), sensitivity = 97%, and specificity = 88%. Men with abnormal semen parameters have decreased levels of PON-1 activity in their seminal plasma. This may play an important role in the pathogenesis of male-factor subfertility.
氧化应激与男性不育的发病机制有关。对氧磷酶-1（PON-1）是一种与高密度脂蛋白相关的抗氧化酶，可防止低密度脂蛋白的氧化修饰。我们在该研究中的目的是调查：1）不育男性精液中的PON-1活性；2）精液PON-1活性是否与精液参数存在任何关系。该研究纳入了28名特发性不育男性、32名精液参数异常的不育男性以及30名有生育能力的男性志愿者。采用分光光度法测定精液PON-1活性。采用比色法测定精液总抗氧化状态（TAS）和总氧化状态（TOS）。氧化应激指数（OSI）计算为（[TOS/TAS]×100）。精液参数异常的不育男性的TOS和OSI显著更高，而PON-1活性和TAS显著低于特发性不育男性和有生育能力的供者。在整个研究组中，PON-1活性还与精子浓度（r = 0.68，P < 0.0001）、活力（r = 0.58，P < 0.0001）和形态（r = 0.62，P < 0.0001）密切相关。受试者工作特征曲线分析显示，PON-1活性对男性因素不育具有较高的诊断价值，曲线下面积为0.95（95%置信区间 = 0.89 - 1.01），敏感性 = 97%，特异性 = 88%。精液参数异常的男性其精浆中PON-1活性水平降低。这可能在男性因素不育的发病机制中起重要作用。
