Division of Biomaterials and Tissue Engineering, Department of Clinical Research, UCL Eastman Dental Institute, University College London, London, UK.
Acta Biomater. 2013 Jan;9(1):4796-805. doi: 10.1016/j.actbio.2012.09.008. Epub 2012 Sep 14.
The adult periodontal ligament (PDL) is considered to contain progenitor cells that are involved in the healing of periodontal wounds. Treatment with enamel matrix derivative (EMD), a heat-treated preparation derived from enamel matrix proteins (EMPs), has been shown to be of some clinical benefit in eliciting periodontal regeneration in vivo. Although there is extensive information available about the effects of EMD on periodontal regeneration, the precise influence of this material on alveolar bone and the formation of blood vessels and proprioceptive sensory nerves, prominent features of functionally active periodontal tissue, remain unclear. The aim of the present study was therefore to examine the effects of EMD on the ability of human periodontal ligament cells (HPCs) to undergo multi-lineage differentiation in vitro. Our results showed that HPCs treated with EMD under non-selective growth conditions did not show any evidence of osteogenic, adipogenic, chondrogenic, neovasculogenic, neurogenic and gliogenic "terminal" differentiation. In contrast, under selective lineage-specific culture conditions, EMD up-regulated osteogenic, chondrogenic and neovasculogenic genes and "terminal" differentiation, but suppressed adipogenesis, neurogenesis and gliogenesis. These findings thus demonstrate for the first time that EMD can differentially modulate the multi-lineage differentiation of HPCs in vitro.
成人牙周韧带（PDL）被认为包含参与牙周伤口愈合的祖细胞。已经证明，使用釉基质衍生物（EMD）治疗，这是一种源自釉基质蛋白（EMP）的热处理制剂，在体内引发牙周再生方面具有一定的临床益处。尽管有大量关于 EMD 对牙周再生影响的信息，但这种材料对牙槽骨的形成以及血管和本体感觉神经的形成（牙周组织功能活跃的突出特征）的确切影响仍不清楚。因此，本研究旨在探讨 EMD 对人牙周韧带细胞（HPC）体外多能性分化能力的影响。我们的结果表明，在非选择性生长条件下用 EMD 处理的 HPC 没有表现出任何成骨、成脂、软骨形成、新生血管形成、神经形成和神经胶质形成的“终末”分化的证据。相比之下，在选择性谱系特异性培养条件下，EMD 上调了成骨、软骨形成和新生血管形成基因以及“终末”分化，但抑制了成脂、神经发生和神经胶质形成。这些发现首次表明，EMD 可以在体外差异调节 HPC 的多能性分化。
