Wang Zhongshan, Feng Zhihong, Wu Guofeng, Bai Shizhu, Dong Yan, Zhao Yimin
State Key Laboratory of Military Stomatology, Department of Prosthetic Dentistry, School of Stomatology, The Fourth Military Medical University, No. 145 West Changle Road, Xi'an 710032, China.
State Key Laboratory of Military Stomatology, Department of Prosthetic Dentistry, School of Stomatology, The Fourth Military Medical University, No. 145 West Changle Road, Xi'an 710032, China.
Colloids Surf B Biointerfaces. 2016 May 1;141:102-111. doi: 10.1016/j.colsurfb.2016.01.036. Epub 2016 Jan 22.
Numerous preclinical and clinical studies have focused on the periodontal regenerative functions of enamel matrix derivative (EMD), a heat-treated preparation derived from enamel matrix proteins (EMPs) of developing porcine teeth. In this study, periodontal ligament (PDL) stem cells (PDLSCs) were isolated, and the effects of EMD on the extracorporeal induction process and the characteristics of PDLSC sheets were investigated for their potential as a more effective stem-cell therapy. EMD-enhanced cell sheets could be induced by complete medium supplemented with 50 μg/mL vitamin C and 100 μg/mL EMD. The EMD-enhanced cell sheets appeared thicker and more compact than the normal PDLSC sheets, demonstrated more layers of cells (3-7 layers), secreted richer extracellular matrix (ECM), showed varying degrees of increases in mRNA expression of periodontal tissue-specific genes (COL I, POSTN), calcification-related genes (RUNX2, OPN, OCN) and a cementum tissue-specific gene (CAP), and possessed a better mineralization ability in terms of osteogenic differentiation in vitro. These EMD-enhanced cell sheets may represent a potential option for stem-cell therapy for PDL regeneration.
众多临床前和临床研究都聚焦于釉基质衍生物（EMD）的牙周再生功能，EMD是一种由发育中的猪牙釉基质蛋白（EMP）经热处理制备而成的物质。在本研究中，分离出了牙周膜（PDL）干细胞（PDLSCs），并研究了EMD对体外诱导过程以及PDLSC片层特性的影响，以探究其作为一种更有效干细胞疗法的潜力。添加50μg/mL维生素C和100μg/mL EMD的完全培养基可诱导出EMD增强的细胞片层。EMD增强的细胞片层比正常PDLSC片层更厚且更致密，显示出更多细胞层（3 - 7层），分泌更丰富的细胞外基质（ECM），牙周组织特异性基因（COL I、POSTN）、钙化相关基因（RUNX2、OPN、OCN）和牙骨质组织特异性基因（CAP）的mRNA表达呈现不同程度的增加，并且在体外成骨分化方面具有更好的矿化能力。这些EMD增强的细胞片层可能代表了用于PDL再生的干细胞疗法的一种潜在选择。
