正常形态精子-透明带结合正常的不育男性中，蛋白激酶 A 和 C 途径缺陷是导致透明带诱导顶体反应障碍的常见原因。

Defective protein kinase A and C pathways are common causes of disordered zona pellucida (ZP)--induced acrosome reaction in normozoospermic infertile men with normal sperm-ZP binding.

机构信息

Melbourne IVF, East Melbourne, Victoria, Australia; Department of Obstetrics and Gynaecology, Royal Women's Hospital, University of Melbourne, Parkville, Victoria, Australia.

Melbourne IVF, East Melbourne, Victoria, Australia.

出版信息

Fertil Steril. 2013 Jan;99(1):86-91. doi: 10.1016/j.fertnstert.2012.08.040. Epub 2012 Sep 15.

DOI:10.1016/j.fertnstert.2012.08.040

PMID:22985948

Abstract

OBJECTIVE

To determine association between defective protein kinases C (PKC) and A (PKA) and disordered zona pellucida (ZP)-induced acrosome reaction (DZPIAR) in normozoospermic infertile men with normal sperm-ZP binding.

DESIGN

Sperm from DZPIAR infertile men were treated without (control) or with (test) phorbol myristate acetate (PMA, PKC activator) or dibutyryl cyclic AMP (dbcAMP, PKA activator) under in vitro standard culture condition. The ZP-induced AR was assessed and compared between control and test.

SETTING

Public and private hospital-based clinical assisted reproduction technology (ART) centers.

PATIENT(S): A total of 51 DZPIAR infertile men were involved in this study.

INTERVENTION(S): None.

MAIN OUTCOMES MEASURE(S): Sperm-ZP binding and the ZP-induced IAR.

RESULT(S): Both PMA and dbcAMP enhanced ZP-induced AR up to a normal level (≥25%) in some subjects with DZPIAR: 29 (57%) with PMA and 27 (53%) with dbcAMP. Overall 35 (69%) had the ZP-induced AR enhanced to normal by PMA or dbcAMP but 16 (31%) had little or no response to either agent. Fourteen men responded to the two activators differently: 8 effective only with PMA and 6 effective only with dbcAMP.

CONCLUSION(S): Defective upstream of PKC and PKA pathways are highly associated with disordered ZPIAR in normozoospermic infertile men with normal sperm-ZP binding.

摘要

目的

确定在具有正常精子-ZP 结合的正常精子不育男性中，缺陷蛋白激酶 C（PKC）和 A（PKA）与异常透明带（ZP）诱导顶体反应（DZPIAR）之间的关联。

设计

在体外标准培养条件下，用佛波醇肉豆蔻酸酯（PKC 激活剂）或二丁酰环 AMP（PKA 激活剂）处理 DZPIAR 不育男性的精子，不处理（对照）或处理（试验）。评估并比较对照和试验之间的 ZP 诱导的 AR。

地点

公立和私立医院为基础的临床辅助生殖技术（ART）中心。

患者

共有 51 名 DZPIAR 不育男性参与了这项研究。

干预

无。

主要观察指标

精子-ZP 结合和 ZP 诱导的 IAR。

结果

PKM 和 dbcAMP 均增强了 DZPIAR 中某些受试者的 ZP 诱导的 AR，达到正常水平（≥25%）：29 名（57%）用 PMA，27 名（53%）用 dbcAMP。总体而言，有 35 名（69%）的人通过 PMA 或 dbcAMP 将 ZP 诱导的 AR 增强至正常水平，但有 16 名（31%）对两者的反应很小或没有。14 名男性对两种激活剂的反应不同：8 名仅对 PMA 有效，6 名仅对 dbcAMP 有效。