Kızılay Fuat, Altay Barış
Department of Urology, Ege University School of Medicine, İzmir, Turkey.
Turk J Urol. 2017 Dec;43(4):393-400. doi: 10.5152/tud.2017.96646. Epub 2017 Dec 1.
Conventional semen analysis solely is not completely adequate to predict pregnancy outcomes. Therefore, advanced sperm function tests have been developed and introduced to clinical practice. These tests use different methods and techniques to evaluate different stages of fertilization steps. In this review, we reported some commonly used sperm function tests: sperm penetration assay, sperm-zona pellucida binding test (hemizona assay), acrosomal reaction test, hyaluronan binding test, hypo-osmotic swelling test, magnetic-activated cell sorting and zeta sperm selection. We discussed the literature concerning these tests, the utilization techniques and also purpose and mechanism of each test. We emphasized the importance of sperm function tests in predicting in vitro fertilization and pregnancy outcomes and in the management of infertile couples and also the limitations of these tests. Along with improvements in molecular biology techniques, we believe that more applicative and beneficial tests will be developed in the near future.
单纯的传统精液分析不足以完全预测妊娠结局。因此，先进的精子功能检测方法已被开发并引入临床实践。这些检测使用不同的方法和技术来评估受精过程的不同阶段。在本综述中，我们报告了一些常用的精子功能检测方法：精子穿透试验、精子-透明带结合试验（半透明带试验）、顶体反应试验、透明质酸结合试验、低渗肿胀试验、磁激活细胞分选和ζ精子筛选。我们讨论了有关这些检测的文献、使用技术以及每项检测的目的和机制。我们强调了精子功能检测在预测体外受精和妊娠结局以及不育夫妇管理中的重要性，以及这些检测的局限性。随着分子生物学技术的进步，我们相信在不久的将来会开发出更多实用且有益的检测方法。
