Department of Environmental Microbiology and Biotechnology, Institute of Ecology and Environmental Protection, Nicolaus Copernicus University, Gagarina 9, Toruń, Poland.
Curr Microbiol. 2012 Dec;65(6):776-83. doi: 10.1007/s00284-012-0230-4. Epub 2012 Sep 18.
This paper presents the results of studies on the activity of extra-cellular enzymes in soil-willow vegetation filter soil which is used in the post-treatment of household sewage in an onsite wastewater treatment system located in central Poland. Wastewater is discharged from the detached house by gravity into the onsite wastewater treatment system. It flows through a connecting pipe into a single-chamber septic tank and is directed by the connecting pipe to a control well to be further channelled in the soil-willow filter by means of a subsurface leaching system. Soil samples for the studies were collected from two depths of 5 cm and 1 m from three plots: close to the wastewater inflow, at mid-length of the plot and close to its terminal part. Soil samples were collected from May to October 2009. The activity of the extra-cellular enzymes was assayed by the fluorometric method using 4-methylumbelliferyl and 7-amido-4-methylcoumarin substrate. The ranking of potential activity of the assayed enzymes was the same at 5 cm and 1 m soil depths, i.e. esterase > phosphmomoesterase > leucine-aminopeptidase > β-glucosidase > α-glucosidase. The highest values of enzymatic activity were recorded in the surface layer of the soil at the wastewater inflow and decreased with increasing distance from that point.
本文介绍了在波兰中部的一个现场污水处理系统中，用于家庭污水后处理的柳树植被过滤土中细胞外酶活性的研究结果。污水通过重力从独立式住宅排入现场污水处理系统。它通过连接管流入单室化粪池，并通过连接管引导至控制井，通过地下渗滤系统进一步进入柳树过滤器。研究用的土壤样本取自三个地块的 5 厘米和 1 米两个深度：靠近污水入口、地块中间和靠近末端。土壤样本于 2009 年 5 月至 10 月采集。使用 4-甲基伞形酮和 7-氨基-4-甲基香豆素作为底物，通过荧光法测定细胞外酶的活性。在 5 厘米和 1 米土壤深度处，测定酶的潜在活性的排序相同，即酯酶>磷酸酯酶>亮氨酸氨基肽酶>β-葡萄糖苷酶>α-葡萄糖苷酶。在污水入口处的土壤表层记录到最高的酶活性值，并随着与该点的距离增加而降低。
