European Regional Centre for Ecohydrology of the Polish Academy of Sciences, Lodz, Poland.
Department of Plant Physiology and Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
Sci Total Environ. 2017 May 15;586:66-75. doi: 10.1016/j.scitotenv.2017.02.012. Epub 2017 Feb 12.
The aim of the study was to determine the impact of sewage sludge from three wastewater treatment plants of different sizes (small, medium and large) applied in two doses (3 and 9 tons per hectare) on soil properties, determined as the content of organic carbon and humus fractions, bacterial abundance, phytotoxicity and PCDD/PCDF TEQ concentrations. The study also evaluated the impact of this sewage sludge on the biometric and physiological parameters and detoxification reaction of willow (Salix sp.) as a typical crop used for the remediation of soil following sludge application. The cultivation of willow on soil treated with sludge was found to result in a gradual increase of humus fractions, total organic carbon content and bacterial abundance as well as soil properties measured using Lepidium sativum. However, it also produced an initial increase of soil phytotoxicity, indicated by Sinapis alba and Sorghum sacharatum, and PCDD/PCDF Toxic Equivalent (TEQ) concentrations, which then fell during the course of the experiment, particularly in areas planted by willow. Although the soil phytotoxicity and PCDD/PCDF TEQ content of the sewage sludge-amended soil initially increased, sludge application was found to have a positive influence on willow, probably due to its high nutrient and carbon content. The obtained results reveal increases in willow biomass, average leaf surface area and leaf length as well as chlorophyll a+b content. Moreover, a strong decline was found in the activity of the detoxifying enzyme glutathione S-transferase (GSTs), a multifunctional enzyme involved in the metabolism of xenobiotics in plants, again demonstrating the used sludge had a positive influence on willow performance.
本研究旨在确定来自三个不同规模（小、中、大）的污水处理厂的污水污泥以两种剂量（每公顷 3 吨和 9 吨）施用于土壤后对土壤性质（有机碳和腐殖质含量、细菌丰度、植物毒性和 PCDD/PCDF TEQ 浓度）的影响。该研究还评估了这种污水污泥对柳树（柳树属）的生物计量和生理参数以及解毒反应的影响，柳树是一种典型的用于污泥施用后土壤修复的作物。研究发现，柳树在污泥处理过的土壤上的种植导致腐殖质含量、总有机碳含量和细菌丰度逐渐增加，同时使用蕺菜和甜高粱测量的土壤性质也得到了增加。然而，它也导致了土壤植物毒性的初始增加，表明白芥和高粱，以及 PCDD/PCDF 毒性当量（TEQ）浓度，然后在实验过程中下降，特别是在柳树种植的地区。尽管污水污泥中添加的土壤植物毒性和 PCDD/PCDF TEQ 含量最初增加，但发现污泥的应用对柳树有积极影响，这可能是由于其高营养和碳含量。研究结果表明柳树生物量、平均叶片面积和叶片长度以及叶绿素 a+b 含量增加。此外，还发现解毒酶谷胱甘肽 S-转移酶（GSTs）的活性强烈下降，GSTs 是一种参与植物中外源物质代谢的多功能酶，这再次表明所使用的污泥对柳树的性能有积极影响。
