Department of Head and Neck Surgery, A. C. Camargo Cancer Hospital, São Paulo, São Paulo, Brazil; Ludwig Institute for Cancer Research, São Paulo Branch, São Paulo, Brazil.
Head Neck. 2013 Oct;35(10):1475-81. doi: 10.1002/hed.23169. Epub 2012 Sep 18.
Oral cancer is the most common subset of head and neck squamous cell carcinomas (HNSCC). These tumors often have an aggressive clinical outcome hallmarked by a propensity for local invasion and regional nodal metastasis. Upregulated genes could be useful as markers for diagnosis, prognosis, and as new drug targets for these tumors.
To identify upregulated genes in oral squamous cell carcinomas (OSSCs), we examined the ORESTES public database and used a quantitative reverse transcription-polymerase chain reaction (qRT-PCR) approach to determine the expression level of selected genes in tumor samples.
The ORESTES data mining analysis indicated 40 upregulated genes in HNSCC. Nine of these candidate genes were selected for further qRT-PCR validation and 3 of them (ALDOA, AHSA1, and POLQ) were frequently found upregulated in OSCC samples, which may indicate an association of these genes with the carcinogenesis process in this tumor site and they can constitute potential new targets for therapy.
口腔癌是头颈部鳞状细胞癌（HNSCC）中最常见的亚型。这些肿瘤通常具有侵袭性的临床结局，其特征是局部侵袭和区域淋巴结转移的倾向。上调的基因可以作为这些肿瘤的诊断、预后和新药靶点的标志物。
为了鉴定口腔鳞状细胞癌（OSSCs）中上调的基因，我们检查了 ORESTES 公共数据库，并使用定量逆转录聚合酶链反应（qRT-PCR）方法来确定选定基因在肿瘤样本中的表达水平。
ORESTES 数据分析表明 HNSCC 中有 40 个上调基因。选择其中的 9 个候选基因进行进一步的 qRT-PCR 验证，其中 3 个（ALDOA、AHSA1 和 POLQ）在 OSCC 样本中经常上调，这可能表明这些基因与该肿瘤部位的癌变过程有关，它们可以构成潜在的新治疗靶点。
