口腔鳞状细胞癌中线粒体拷贝数减少。
Decreased mitochondrial copy numbers in oral squamous cell carcinoma.
作者信息
Takeda Daisuke, Hasegawa Takumi, Ueha Takeshi, Sakakibara Akiko, Kawamoto Teruya, Minamikawa Tsutomu, Sakai Yoshitada, Komori Takahide
机构信息
Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
Department of Rehabilitation Medicine, Kobe University Graduate School of Medicine, Kobe, Japan.
出版信息
Head Neck. 2016 Aug;38(8):1170-5. doi: 10.1002/hed.24194. Epub 2016 Apr 15.
BACKGROUND
Mitochondrial dysfunction and altered respiration have long been suspected to affect the development and progression of cancer. Although quantitative changes in mitochondrial DNA (mtDNA) have been reported in head and neck squamous cell carcinoma (SCC), differences in mtDNA copy numbers between normal and cancerous tissues from same patients have not been assessed.
METHODS
We compared mtDNA copy numbers and expressions of peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α) and mitochondrial transcription factor A (TFAM) between normal mucous membrane and cancerous tissues resected from 35 patients with oral SCC, using TaqMan quantitative real-time polymerase chain reaction (PCR) and immunohistochemical staining.
RESULTS
We found mtDNA copy numbers and expressions of PGC-1α and TFAM were decreased in cancerous tissues compared with normal tissues from the same patients.
CONCLUSION
The PGC-1α-TFAM mitochondrial pathway may be associated with malignant potential in human oral SCC, and could be an attractive therapeutic target. © 2016 Wiley Periodicals, Inc. Head Neck 38:1170-1175, 2016.
背景
长期以来，人们一直怀疑线粒体功能障碍和呼吸改变会影响癌症的发生发展。虽然在头颈部鳞状细胞癌（SCC）中已报道线粒体DNA（mtDNA）存在定量变化，但尚未评估同一患者正常组织与癌组织之间mtDNA拷贝数的差异。
方法
我们采用TaqMan定量实时聚合酶链反应（PCR）和免疫组织化学染色，比较了35例口腔SCC患者切除的正常黏膜组织与癌组织之间的mtDNA拷贝数以及过氧化物酶体增殖物激活受体γ共激活因子-1α（PGC-1α）和线粒体转录因子A（TFAM）的表达。
结果
我们发现，与同一患者的正常组织相比，癌组织中的mtDNA拷贝数以及PGC-1α和TFAM的表达均降低。
结论
PGC-1α-TFAM线粒体途径可能与人类口腔SCC的恶性潜能相关，并且可能是一个有吸引力的治疗靶点。©2016威利期刊公司。《头颈》38:1170 - 1175，2016年。
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