Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 N° 3, B1904AMA La Plata, Argentina.
Environ Toxicol. 2014 Aug;29(8):884-92. doi: 10.1002/tox.21816. Epub 2012 Sep 15.
The in-vitro effects of flurochloridone and its formulations Twin Pack Gold® (25% a.i.) and Rainbow® (25% a.i.) were evaluated in Chinese Hamster Ovary K1 (CHO-K1) cells. The cytokinesis-block micronucleus cytome (CBMN-cyt) and single-cell gel electrophoresis (SCGE) assays were used. The activities were tested within the range of final concentrations of 0.25-15 μg flurochloridone/mL. The results demonstrated that both the flurochloridone and Rainbow® were not able to induce micronuclei (MN). On the other hand, Twin Pack Gold® only increased the frequency of MN at 5 μg/mL. Furthermore, 10 and 15 μg/mL of both formulations resulted in a cellular cytotoxicity demonstrated by alterations in the nuclear division index and cellular death. SCGE assay appeared to be a more sensitive bioassay for detecting primary DNA strand breaks at lower concentrations of flurochloridone than MN did. A marked increase in the genetic damage index was observed when 5 and 15 μg/mL of both flurochloridone and Rainbow® but only when 15 μg/mL of Twin Pack Gold® were used. This is the first report demonstrating that flurochloridone and its two commercial formulations are able to induce single-strand DNA breaks in vitro on mammalian cells.
氟氯氰菊酯及其复配制剂 Twin Pack Gold®（25%有效成分）和 Rainbow®（25%有效成分）在体外对中国仓鼠卵巢 K1（CHO-K1）细胞的作用进行了评估。采用细胞有丝分裂阻断微核细胞遗传学试验（CBMN-cyt）和单细胞凝胶电泳（SCGE）检测方法。在终浓度为 0.25-15μg 氟氯氰菊酯/mL 的范围内进行了活性测试。结果表明，氟氯氰菊酯和 Rainbow®均不能诱导微核（MN）。另一方面，Twin Pack Gold®仅在 5μg/mL 时增加 MN 的频率。此外，两种制剂的 10 和 15μg/mL 浓度均导致核分裂指数和细胞死亡改变，表现出细胞毒性。SCGE 试验似乎比 MN 更能灵敏地检测到较低浓度氟氯氰菊酯的原发性 DNA 链断裂。当使用 5 和 15μg/mL 的氟氯氰菊酯和 Rainbow®时，遗传损伤指数明显增加，但仅当使用 15μg/mL 的 Twin Pack Gold®时才增加。这是首次报道表明氟氯氰菊酯及其两种商业制剂能够在体外诱导哺乳动物细胞的单链 DNA 断裂。
