二甲亚砜法（DMMB）测定硫酸化糖胺聚糖。
The Dimethylmethylene Blue Assay (DMMB) for the Quantification of Sulfated Glycosaminoglycans.
机构信息
AO Research Institute Davos, Davos Platz, Switzerland.
Institute for Biomechanics, ETH Zurich, Zurich, Switzerland.
出版信息
Methods Mol Biol. 2023;2598:115-121. doi: 10.1007/978-1-0716-2839-3_9.
PMID:36355288
Abstract
The 1,9-dimethylmethylene blue (DMMB) assay enables the detection of sulfated glycosaminoglycans (sGAGs). This assay can be used to quickly quantify the sGAG content in a large number of samples using spectrophotometry. While this widespread assay appears straightforward, there are certain pitfalls that need to be considered.
摘要
1,9-二甲基亚甲蓝 (DMMB) 法可用于检测硫酸化糖胺聚糖 (sGAG)。该方法可用于使用分光光度法快速定量大量样品中的 sGAG 含量。虽然这种广泛使用的方法看起来很简单，但需要考虑一些潜在问题。
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