• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

二甲亚砜法（DMMB）测定硫酸化糖胺聚糖。

The Dimethylmethylene Blue Assay (DMMB) for the Quantification of Sulfated Glycosaminoglycans.

机构信息

AO Research Institute Davos, Davos Platz, Switzerland.

Institute for Biomechanics, ETH Zurich, Zurich, Switzerland.

出版信息

Methods Mol Biol. 2023;2598:115-121. doi: 10.1007/978-1-0716-2839-3_9.

DOI:10.1007/978-1-0716-2839-3_9
PMID:36355288
Abstract

The 1,9-dimethylmethylene blue (DMMB) assay enables the detection of sulfated glycosaminoglycans (sGAGs). This assay can be used to quickly quantify the sGAG content in a large number of samples using spectrophotometry. While this widespread assay appears straightforward, there are certain pitfalls that need to be considered.

摘要

1,9-二甲基亚甲蓝 (DMMB) 法可用于检测硫酸化糖胺聚糖 (sGAG)。该方法可用于使用分光光度法快速定量大量样品中的 sGAG 含量。虽然这种广泛使用的方法看起来很简单，但需要考虑一些潜在问题。

相似文献

1
The Dimethylmethylene Blue Assay (DMMB) for the Quantification of Sulfated Glycosaminoglycans.二甲亚砜法（DMMB）测定硫酸化糖胺聚糖。
Methods Mol Biol. 2023;2598:115-121. doi: 10.1007/978-1-0716-2839-3_9.
2
Assessment of three variations of the 1,9-dimethylmethylene blue assay for measurement of sulfated glycosaminoglycan concentrations in equine synovial fluid.评估用于测量马滑液中硫酸化糖胺聚糖浓度的1,9 - 二甲基亚甲蓝测定法的三种变体。
Am J Vet Res. 2003 Jul;64(7):900-6. doi: 10.2460/ajvr.2003.64.900.
3
Fact versus artifact: avoiding erroneous estimates of sulfated glycosaminoglycan content using the dimethylmethylene blue colorimetric assay for tissue-engineered constructs.事实与假象：使用二甲基亚甲基蓝比色法检测组织工程构建体时避免硫酸化糖胺聚糖含量的错误估计
Eur Cell Mater. 2015 Apr 19;29:224-36; discussion 236. doi: 10.22203/ecm.v029a17.
4
Development of a solid-phase assay for measurement of sulfated glycosaminoglycan concentrations in equine synovial fluid.用于测量马滑液中硫酸化糖胺聚糖浓度的固相分析方法的开发。
Am J Vet Res. 2003 Jul;64(7):894-9. doi: 10.2460/ajvr.2003.64.894.
5
Studies on the quantification of proteoglycans by the dimethylmethylene blue dye-binding method. Specificity, quantitation in synovial lavage fluid, and automation.用二甲基亚甲基蓝染料结合法对蛋白聚糖进行定量研究。特异性、滑膜灌洗液中的定量以及自动化。
Connect Tissue Res. 1992;28(4):317-24. doi: 10.3109/03008209209016823.
6
The basis and applicability of the dimethylmethylene blue binding assay for sulfated glycosaminoglycans.用于硫酸化糖胺聚糖的二甲基亚甲基蓝结合测定法的基础及适用性。
Connect Tissue Res. 1988;17(1):23-32. doi: 10.3109/03008208808992791.
7
[Indirect spectrophotometric microdetermination of sulfated glycosaminoglycans with dimethylmethylene blue].[用二甲基亚甲基蓝间接分光光度法微量测定硫酸化糖胺聚糖]
Z Med Lab Diagn. 1989;30(3):180-5.
8
Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue.使用二甲基亚甲基蓝改进硫酸化糖胺聚糖的定量和鉴别
Biochim Biophys Acta. 1986 Sep 4;883(2):173-7. doi: 10.1016/0304-4165(86)90306-5.
9
A direct spectrophotometric microassay for sulfated glycosaminoglycans in cartilage cultures.软骨培养物中硫酸化糖胺聚糖的直接分光光度微量测定法。
Connect Tissue Res. 1982;9(4):247-8. doi: 10.3109/03008208209160269.
10
Automation of 1,9-dimethylmethylene blue dye-binding assay for sulfated glycosaminoglycans with application to cartilage microcultures.用于硫酸化糖胺聚糖的1,9 - 二甲基亚甲基蓝染料结合测定法的自动化及其在软骨微培养中的应用。
Anal Biochem. 1985 Sep;149(2):543-8. doi: 10.1016/0003-2697(85)90611-6.

引用本文的文献

1
Quantitative HILIC-Q-TOF-MS Analysis of Glycosaminoglycans and Non-reducing End Carbohydrate Biomarkers via Glycan Reductive Isotopic Labeling.通过聚糖还原同位素标记对糖胺聚糖和非还原端碳水化合物生物标志物进行定量亲水相互作用色谱-四极杆飞行时间质谱分析。
Anal Chem. 2025 Aug 19;97(32):17490-17500. doi: 10.1021/acs.analchem.5c02338. Epub 2025 Aug 6.
2
Skeletal dysplasia-causing mutations in TRPV4 alter the chondrocyte transcriptomic response to mechanical loading.导致骨骼发育异常的TRPV4突变会改变软骨细胞对机械负荷的转录组反应。
Am J Physiol Cell Physiol. 2025 Apr 1;328(4):C1135-C1149. doi: 10.1152/ajpcell.01066.2024. Epub 2025 Feb 28.
3

本文引用的文献

1
A method for characterising human intervertebral disc glycosaminoglycan disaccharides using liquid chromatography-mass spectrometry with multiple reaction monitoring.采用液相色谱-串联质谱多重反应监测法对人椎间盘中糖胺聚糖二糖进行特征分析。
Eur Cell Mater. 2018 Feb 22;35:117-131. doi: 10.22203/eCM.v035a09.
2
Fact versus artifact: avoiding erroneous estimates of sulfated glycosaminoglycan content using the dimethylmethylene blue colorimetric assay for tissue-engineered constructs.事实与假象：使用二甲基亚甲基蓝比色法检测组织工程构建体时避免硫酸化糖胺聚糖含量的错误估计
Eur Cell Mater. 2015 Apr 19;29:224-36; discussion 236. doi: 10.22203/ecm.v029a17.
3
Synovial joints were present in the common ancestor of jawed fish but lacking in jawless fish.
滑膜关节存在于有颌鱼类的共同祖先中，但在无颌鱼类中不存在。
PLoS Biol. 2025 Feb 25;23(2):e3002990. doi: 10.1371/journal.pbio.3002990. eCollection 2025 Feb.
4
Phenotypic alterations in articulating joint cells: Role of mechanically loaded MSC secretome.关节细胞的表型改变：机械加载的间充质干细胞分泌组的作用。
Heliyon. 2025 Jan 25;11(3):e42234. doi: 10.1016/j.heliyon.2025.e42234. eCollection 2025 Feb 15.
5
Establishment of an ex vivo cartilage damage model by combined collagenase treatment and mechanical loading.通过胶原酶处理和机械加载联合建立体外软骨损伤模型。
Arthritis Res Ther. 2025 Feb 11;27(1):30. doi: 10.1186/s13075-025-03499-7.
6
The Expression of Toll-like Receptors in Cartilage Endplate Cells: A Role of Toll-like Receptor 2 in Pro-Inflammatory and Pro-Catabolic Gene Expression.软骨终板细胞中 Toll 样受体的表达：Toll 样受体 2 在促炎和促分解代谢基因表达中的作用。
Cells. 2024 Aug 23;13(17):1402. doi: 10.3390/cells13171402.
7
The Role of the Gut Microbiota in Sanfilippo Syndrome's Physiopathology: An Approach in Two Affected Siblings.肠道微生物群在 Sanfilippo 综合征发病机制中的作用：两例患病同胞的研究方法。
Int J Mol Sci. 2024 Aug 14;25(16):8856. doi: 10.3390/ijms25168856.
8
The chondrocyte "mechanome": Activation of the mechanosensitive ion channels TRPV4 and PIEZO1 drives unique transcriptional signatures.软骨细胞的“力学系统”：机械敏感离子通道 TRPV4 和 PIEZO1 的激活驱动独特的转录特征。
FASEB J. 2024 Jul 15;38(13):e23778. doi: 10.1096/fj.202400883R.
9
Type II collagen scaffolds repair critical-sized osteochondral defects under induced conditions of osteoarthritis in rat knee joints via inhibiting TGF-β-Smad1/5/8 signaling pathway.II型胶原支架通过抑制TGF-β-Smad1/5/8信号通路修复大鼠膝关节骨关节炎诱导条件下的临界尺寸骨软骨缺损。
Bioact Mater. 2024 Feb 16;35:416-428. doi: 10.1016/j.bioactmat.2024.02.008. eCollection 2024 May.
10
Degradation Product of Sea Cucumber Polysaccharide by Dielectric Barrier Discharge Enhanced the Migration of Macrophage In Vitro.介电屏障放电处理的海参多糖降解产物增强巨噬细胞体外迁移能力
Foods. 2023 Nov 10;12(22):4079. doi: 10.3390/foods12224079.
Performance of different screening methods for the determination of urinary glycosaminoclycans.
不同筛查方法在尿氨基己糖苷类检测中的性能比较。
Clin Chem Lab Med. 2013 Feb;51(2):347-50. doi: 10.1515/cclm-2012-0471.
4
Measurement of glycosaminoglycan release from cartilage explants.软骨外植体中糖胺聚糖释放量的测定。
Methods Mol Med. 2007;135:201-9. doi: 10.1007/978-1-59745-401-8_12.
5
Urinary glycosaminoglycan excretion quantified by an automated semimicro method in specimens conveniently transported from around the globe.采用自动半微量法对从全球各地便捷运输而来的标本中的尿糖胺聚糖排泄量进行定量分析。
Mol Genet Metab. 2002 Jan;75(1):56-64. doi: 10.1006/mgme.2001.3271.
6
A direct spectrophotometric microassay for sulfated glycosaminoglycans in cartilage cultures.软骨培养物中硫酸化糖胺聚糖的直接分光光度微量测定法。
Connect Tissue Res. 1982;9(4):247-8. doi: 10.3109/03008208209160269.
7
A new basic metachromatic dye, I:9-dimethyl methylene blue.
Histochem J. 1969 Feb;1(3):199-204. doi: 10.1007/BF01081408.
8
Determination of sulphated disaccharides from chondroitin sulphates by high-performance liquid chromatography.采用高效液相色谱法测定硫酸软骨素中的硫酸化二糖。
J Chromatogr. 1979 Apr 1;171:339-44. doi: 10.1016/s0021-9673(01)95313-0.