A型产气荚膜梭菌肠毒素的氨基酸序列

The amino acid sequence of the enterotoxin from Clostridium perfringens type A.

作者信息

Richardson M, Granum P E

出版信息

FEBS Lett. 1985 Mar 25;182(2):479-84. doi: 10.1016/0014-5793(85)80358-6.

PMID:3920076

Abstract

The amino acid sequence of the enterotoxin from Clostridium perfringens type A was determined by analysis of peptides derived from the protein by digestion with trypsin chymotrypsin, thermolysin, pepsin, a lysine-specific protease. S. aureus V8 protease and a proline-specific protease, and fragments generated by cleavage with cyanogen bromide or by dilute acetic acid in 7 M guanidine HCl. The sequence which is complete except for the definite order of 3 small peptides between residues 88 and 103 consists of 309 amino acids and contains a correction to our preliminary announcement [(1984) FEMS Symp. 24, 329-330].

摘要

通过分析产气荚膜梭菌A型肠毒素经胰蛋白酶、糜蛋白酶、嗜热菌蛋白酶、胃蛋白酶、赖氨酸特异性蛋白酶、金黄色葡萄球菌V8蛋白酶和脯氨酸特异性蛋白酶消化产生的肽段，以及经溴化氰裂解或在7M盐酸胍中用稀醋酸裂解产生的片段，确定了该肠毒素的氨基酸序列。除了88至103位残基之间3个小肽的确定顺序外，完整的序列由309个氨基酸组成，并对我们的初步报告进行了修正[（1984年）FEMS研讨会。24, 329 - 330]。