Cui Guanglin, Yuan Aping, Goll Rasmus, Florholmen Jon
Department of Gastroenterology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Scand J Gastroenterol. 2012 Nov;47(11):1304-12. doi: 10.3109/00365521.2012.725089. Epub 2012 Sep 19.
Interleukin (IL)-17A is an important pro-inflammatory cytokine and involved in the colorectal carcinogenesis. In this study, the authors evaluated the dynamic change of IL-17A expression in the tumor microenvironment throughout the colorectal adenoma-carcinoma sequence.
MATERIALS & METHODS: Using quantitative real-time PCR (polymerase chain reaction) and semi-quantitative immunohistochemistry, the authors examined the expression level of IL-17A in 50 of human colorectal adenoma tissues, 50 of colorectal cancer (CRC) tissues and 15 controls. The relationship between IL-17A expression and clinicopathological parameters throughout the sequence was also evaluated.
The results revealed a step-up increased IL-17A mRNA level throughout the colorectal adenoma-carcinoma sequence, which began to increase in the adenomas and became even higher in the CRCs; notably, the increase of IL-17A mRNA level in the adenomatous tissues was associated with the severity of dysplasia. Immunohistochemical analysis confirmed the real-time PCR results and revealed gradually increasing IL-17A cells in both the stroma and adenomatous/cancerous epithelium. In addition, the quantitative real-time PCR result has also revealed an increased expression of TH17-stimulating factors throughout the sequence.
IL-17A and TH17 are highly activated throughout the colorectal adenoma-carcinoma sequence.
白细胞介素（IL）-17A是一种重要的促炎细胞因子，参与结直肠癌的发生。在本研究中，作者评估了在整个结直肠腺瘤-癌序列中肿瘤微环境中IL-17A表达的动态变化。
作者使用定量实时聚合酶链反应（PCR）和半定量免疫组织化学方法，检测了50例人类结直肠腺瘤组织、50例结直肠癌（CRC）组织和15例对照中IL-17A的表达水平。还评估了整个序列中IL-17A表达与临床病理参数之间的关系。
结果显示，在整个结直肠腺瘤-癌序列中，IL-17A mRNA水平呈逐步升高，在腺瘤中开始升高，在结直肠癌中更高；值得注意的是，腺瘤组织中IL-17A mRNA水平的升高与发育异常的严重程度相关。免疫组织化学分析证实了实时PCR结果，并显示基质和腺瘤/癌上皮中IL-17A阳性细胞逐渐增加。此外，定量实时PCR结果还显示，在整个序列中TH17刺激因子的表达增加。
在整个结直肠腺瘤-癌序列中，IL-17A和TH17被高度激活。
