Department of Obstetrics & Gynecology, Gynecology Division, Faculty of Medicine, University of São Paulo (USP), Av Dr Eneas de Carvalho Aguiar 255, ICHC floor #10, 05403-000 São Paulo, Brazil.
Eur J Obstet Gynecol Reprod Biol. 2012 Dec;165(2):265-70. doi: 10.1016/j.ejogrb.2012.08.022. Epub 2012 Sep 16.
To evaluate the effects of conjugated equine estrogens (CE) alone or in combination with medroxyprogesterone acetate (MPA) on glycosaminoglycans (GAGs) in the cervix and horns of the rat uterus.
Thirty days after ovariectomy, adult rats were randomly divided into four groups: GI, control (treated with drug vehicle); GII, CE (50 μg/kg per day); GIII, MPA (0.2mg/kg per day), and GIV, CE+MPA (doses as in GII and GIII). Drugs and vehicle were given by gavage during 28 days. Afterwards the animals were anesthetized, the cervix and uterine horns were dissected out and the middle portion fixed in 10% formaldehyde solution; other portions were fixed in acetone for histological examination and glycosaminoglycan quantification, respectively. Agarose gel electrophoresis was used for sulfated GAG analyses, and hyaluronic acid was assayed with an ELISA-like method. Statistical analysis was done by the Student's t test and the Tukey-Kramer test (P<0.05).
The cervix and uterine horn structures presented signs of atrophy in the control group (GI). The other groups, mainly groups III and IV, had histological aspects of proliferation. In all groups the concentration of sulfated GAGs (especially dermatan sulfate) was higher than that of non-sulfated GAGs, both in cervix and in uterine horns. Estrogens increased sulfated GAG concentration at the cervix and the horn, whereas in uterine horns the amounts of sulfated GAGs were decreased after estrogens plus MPA treatment. The concentration of hyaluronic acid in uterine horns was higher than in cervices.
The profiling and amounts of glycosaminoglycans in the two portions of the rat uterus are uneven. Dermatan sulfate occurs in higher concentrations in both cervix and uterine horns. Sulfated GAGs in rat cervix were increased by estrogens plus MPA, but were decreased by MPA alone in uterine horns.
评估结合雌激素（CE）单独或与醋酸甲羟孕酮（MPA）联合对大鼠子宫颈和角的糖胺聚糖（GAG）的影响。
卵巢切除 30 天后，成年大鼠被随机分为四组：GI，对照组（用药物载体处理）；GII，CE（50μg/kg/天）；GIII，MPA（0.2mg/kg/天）和 GIV，CE+MPA（剂量与 GII 和 GIII 相同）。28 天内通过灌胃给予药物和载体。之后，将动物麻醉，取出子宫颈和子宫角，并将中间部分固定在 10%甲醛溶液中；其他部分分别固定在丙酮中，用于组织学检查和糖胺聚糖定量。琼脂糖凝胶电泳用于硫酸化 GAG 分析，透明质酸用 ELISA 样方法测定。通过学生 t 检验和 Tukey-Kramer 检验（P<0.05）进行统计分析。
对照组（GI）的子宫颈和子宫角结构出现萎缩迹象。其他组，主要是第三组和第四组，具有增殖的组织学特征。在所有组中，硫酸化 GAG 的浓度（特别是硫酸皮肤素）高于非硫酸化 GAG，无论是在子宫颈还是子宫角。雌激素增加了子宫颈和角的硫酸化 GAG 浓度，而雌激素加 MPA 治疗后子宫角的硫酸化 GAG 量减少。子宫角中透明质酸的浓度高于子宫颈。
大鼠子宫两个部位的糖胺聚糖谱和含量不均匀。硫酸皮肤素在子宫颈和子宫角中都以较高浓度存在。子宫颈的硫酸化 GAG 被雌激素加 MPA 增加，但被 MPA 单独在子宫角中减少。
