University of Vale do Sapucai - UNIVÁS, Gynecology, São Paulo, Brazil.
Climacteric. 2013 Feb;16(1):161-8. doi: 10.3109/13697137.2012.679168. Epub 2012 Jul 4.
To evaluate whether soybean extracts and estrogens present additive effects on adult rat uterus.
Fifty ovariectomized rats were randomly divided into five equal groups of ten animals: Control, treated with vehicle; SE46 and SE120, treated with 46 and 120 mg/kg soybean concentrated extract (SE), respectively; EE, treated with conjugated equine estrogens (CE) 50 μg/kg; SE120 + EE, treated with 50 μg/kg (CE) plus 120 mg/kg SE. The substances were administered daily by gavage for 21 consecutive days. Thereafter the animals were weighed and killed by decapitation; trunk blood was collected for hormone determinations. Uteri were removed immediately and fixed in 10% formaldehyde, followed by dehydration, embedding in paraffin and 6-m sections staining with hematoxylin and eosin for histomorphometric analyses of myometrium and endometrium. After ANOVA analysis of the data, the study was complemented with the Tukey-Kramer test for multiple comparisons.
The concentrated extract of soybean at high concentration (SE 120 kg/mg) and estrogens proved to have a trophic effect on the uterus (endometrium and myometrium) of castrated rats. In groups SE120, EE and SE120 + EE, all morphometric parameters examined (number of glands, eosinophils, blood vessels and the glandular area) were increased. No significant addictive effects of soybean extract plus estrogens were detected in the SE120 + EE group.
Our results indicate that soy extract has a trophic effect on rat uterine structures. Treatment of ovariectomized rats with a concentrated soy extract in combination with conjugated estrogens had no addictive effect on the uterine response.
评估大豆提取物和雌激素对成年大鼠子宫是否具有相加作用。
将 50 只去卵巢大鼠随机分为 5 组，每组 10 只：对照组，给予载体；SE46 和 SE120 组分别给予 46 和 120mg/kg 大豆浓缩提取物（SE）；EE 组给予结合马雌激素（CE）50μg/kg；SE120+EE 组给予 50μg/kg（CE）加 120mg/kg SE。每天通过灌胃给药，连续 21 天。然后称重并断头处死动物；采集血样用于激素测定。立即取出子宫，用 10%甲醛固定，然后进行脱水、石蜡包埋和 6μm 切片，用苏木精和伊红染色，对子宫肌层和子宫内膜进行组织形态计量学分析。对数据进行方差分析后，用 Tukey-Kramer 检验进行多重比较。
高浓度（SE120mg/kg）大豆浓缩提取物和雌激素对去势大鼠的子宫（子宫内膜和子宫肌层）具有营养作用。在 SE120、EE 和 SE120+EE 组中，所有检查的形态计量学参数（腺体数量、嗜酸性粒细胞、血管和腺体面积）均增加。在 SE120+EE 组中，未发现大豆提取物加雌激素有明显的相加作用。
我们的结果表明，大豆提取物对大鼠子宫结构具有营养作用。用浓缩大豆提取物联合结合雌激素治疗去卵巢大鼠对子宫反应没有相加作用。
