Institute of Chemical Technology and Engineering, Poznan University of Technology, Pl. M. Skłodowskiej-Curie 2, 60-965 Poznań, Poland.
N Biotechnol. 2013 Jan 25;30(2):173-82. doi: 10.1016/j.nbt.2012.09.003. Epub 2012 Sep 16.
Surface properties of newly isolated Stenotrophomonas maltophilia strain 6 were tested. The bacteria were stored in two different ways to determine the influence of hydrocarbons and surfactants on surface and enzymatic characteristics of the isolated strain. The influence of surface active agents, natural and synthetic, on membrane's lipid composition and cell surface hydrophobicity (CSH) was investigated. Our results indicate that long-term contact with diesel oil as a hydrophobic sole carbon source leads to the increased enzymatic activity of S. maltophilia strain 6 as well as to modification of fatty acids profiles and its facility to adhere to hydrophobic compounds. Among surfactants there were saponins and Triton X-100 which changed the composition of fatty acids the most, increasing the amount of branched acids. The comparison of fatty acid profiles with CSH of systems with diesel oil, rhamnolipids, saponins and Triton X-100 points out that the growing amount of hydroxy fatty acids corresponds to lower hydrophobicity. Moreover, CSH is a dynamic parameter which can change during cultivation of microorganisms.
对新分离的嗜麦芽寡养单胞菌 6 株的表面特性进行了测试。以两种不同的方式储存细菌，以确定碳氢化合物和表面活性剂对分离菌株表面和酶特性的影响。研究了天然和合成表面活性剂对膜脂组成和细胞表面疏水性（CSH）的影响。我们的结果表明，与柴油等疏碳源长期接触，导致嗜麦芽寡养单胞菌 6 株的酶活性增加，脂肪酸谱发生变化，其对疏水性化合物的粘附能力增强。在表面活性剂中，皂苷和 Triton X-100 改变脂肪酸组成最多，增加了支链酸的数量。将脂肪酸图谱与含柴油、鼠李糖脂、皂苷和 Triton X-100 的系统的 CSH 进行比较，表明羟基脂肪酸的生长量与疏水性降低相对应。此外，CSH 是一个动态参数，在微生物培养过程中会发生变化。
