Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Medicine, Shandong Agricultural University, Shandong, Taian 271018, PR China.
Microb Pathog. 2013 Jan;54:40-5. doi: 10.1016/j.micpath.2012.09.003. Epub 2012 Sep 16.
Multiple infections of Bordetella avium (B. avium) with virus, especially immunosuppressive virus, have become more and more severe in chickens in China. The increasing morbidity and mortality of its complications have amplified concerns about the impact of B. avium on animal health. To evaluate the pathogenicity of B. avium under immunosuppression status, we developed four types of Reticuloendotheliosis virus (REV) infection models. After a comparison of body weight, relative immune organ index, Newcastle disease virus antibody titers and lymphocyte ratio, we chose the early age with low dose infection as our immunosuppressive model. To investigate the pathogenicity of B. avium under this model, a study was completed in which chickens were inoculated with REV-only, B. avium-only, both agents (REV -B. avium) or first inoculated with REV and 5 d later with B. avium (REV/B. avium). Results revealed that antibody titers to B. avium, concentrations of IFN-γ and SIgA were decreased in coinfected chickens when compared to the B. avium-only chickens, but the changing trend was similar between REV/B. avium and B. avium-only groups. Overall, REV did enhance the pathogenicity of B. avium. However, B. avium-only did not cause severe immune dysfunction unless chicks were coinfected with REV. REV preceding infection with B. avium showed mild impairment, which needs further exploration.
在中国，禽鸟感染博德特氏菌（B. avium）与病毒（尤其是免疫抑制性病毒）的多重感染变得越来越严重。其并发症发病率和死亡率的增加加剧了人们对博德特氏菌对动物健康影响的担忧。为了评估免疫抑制状态下博德特氏菌的致病性，我们开发了四种网状内皮组织增生病病毒（REV）感染模型。通过比较体重、相对免疫器官指数、新城疫病毒抗体滴度和淋巴细胞比例，我们选择了早期低剂量感染作为我们的免疫抑制模型。为了研究该模型下博德特氏菌的致病性，我们完成了一项研究，其中鸡只接种了仅 REV、仅 B. avium、两者（REV-B. avium）或先接种 REV 后 5 天接种 B. avium（REV/B. avium）。结果表明，与仅 B. avium 感染的鸡相比，混合感染鸡的 B. avium 抗体滴度、IFN-γ 和 SIgA 浓度降低，但 REV/B. avium 组和仅 B. avium 组的变化趋势相似。总体而言，REV 确实增强了 B. avium 的致病性。然而，除非雏鸡同时感染 REV，否则仅 B. avium 不会导致严重的免疫功能障碍。REV 先于 B. avium 感染显示出轻度损害，这需要进一步探索。
