“标记光”噬菌体作为一种用于检测植物病原体的诊断工具。
"Light-tagged" bacteriophage as a diagnostic tool for the detection of phytopathogens.
机构信息
Guild Associates, Inc., Charleston, SC USA.
出版信息
Bioengineered. 2013 Jan-Feb;4(1):50-4. doi: 10.4161/bioe.22159. Epub 2012 Sep 18.
Abstract
Detection of the phytopathogen Pseudomonas cannabina pv alisalensis, the causal agent of bacterial blight of crucifers is essential for managing this disease. A phage-based diagnostic assay was developed that detects and identifies P. cannabina pv alisalensis from cultures and diseased plant specimens. A recombinant "light-tagged" reporter phage was generated by integrating the luxAB genes into the P. cannabina pv alisalensis phage PBSPCA1 genome. PBSPCA1::luxAB is viable, stable and detects P. cannabina pv alisalensis within minutes and with high sensitivity by conferring a bioluminescent signal. Detection is dependent on cell viability since cells treated with a bactericidal disinfectant are unable to elicit a signal. Importantly, the reporter phage detects P. cannabina pv alisalensis from diseased plant specimens indicating the potential of the diagnostic for disease identification. The reporter phage displays promise for the rapid and specific diagnostic detection of cultivated isolates, and infected plant specimens.
摘要
检测植物病原菌假单胞菌菌核菌 pv alisalensis，这是十字花科细菌性枯萎病的病原体，对于该病的管理至关重要。我们开发了一种基于噬菌体的诊断检测方法，可以从培养物和患病植物标本中检测和鉴定假单胞菌菌核菌 pv alisalensis。通过将 luxAB 基因整合到假单胞菌菌核菌 pv alisalensis 噬菌体 PBSPCA1 基因组中，生成了一种“轻标记”重组报告噬菌体。 PBSPCA1::luxAB 是可行的、稳定的，通过发出生物发光信号，可在数分钟内以高灵敏度检测到假单胞菌菌核菌 pv alisalensis。检测依赖于细胞活力，因为用杀菌消毒剂处理的细胞无法发出信号。重要的是，报告噬菌体可以从患病植物标本中检测到假单胞菌菌核菌 pv alisalensis，这表明该诊断方法有用于疾病鉴定的潜力。报告噬菌体有望快速、特异性地检测培养物分离株和感染植物标本。
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Plant Dis. 2002 Sep;86(9):992-998. doi: 10.1094/PDIS.2002.86.9.992.
2
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