Cintas N A, Koike S T, Bull C T
USDA, ARS, PWA, 1636 E. Alisal Ave., Salinas, CA 93905.
University of California, Cooperative Extension, Salinas 93905.
Plant Dis. 2002 Sep;86(9):992-998. doi: 10.1094/PDIS.2002.86.9.992.
The etiology of three foliar bacterial diseases of crucifers and the relationships between their causal agents were evaluated. Data from LOPAT, carbon utilization tests, and fatty acid analysis indicated that bacterial blights of broccoli and broccoli raab, and leaf spot of broccolini, were caused by strains of Pseudomonas syringae. Data from phage sensitivity, ice nucleation, single carbon source utilization, Polymerase chain reaction using BOXA1R primer (BOX-PCR), and host range analyses were identical for the pathogen causing leaf spot of broccolini and P. syringae pv. maculicola. The broccoli raab and broccoli pathogens infected broccoli raab, all crucifers tested, tomato, and three monocots (California brome, oat, and common timothy). None of the other pathogens tested (P. syringae pv. maculicola, P. syringae pv. tomato, or P. syringae pv. coronafaciens) caused disease on broccoli raab or on both crucifers and monocots. Data from phage sensitivity, ice nucleation, single carbon source utilization, BOX-PCR, and host range analyses were identical for the pathogens from broccoli raab and broccoli, but were different from other pathovars tested, and supported the hypothesis that a new pathovar of P. syringae pv. alisalensis pv. nov. caused a leaf blight on broccoli and broccoli raab.
对十字花科植物三种叶部细菌病害的病因及其病原菌之间的关系进行了评估。来自LOPAT、碳利用测试和脂肪酸分析的数据表明，西兰花和青花菜的细菌性叶枯病以及嫩茎花椰菜的叶斑病是由丁香假单胞菌菌株引起的。来自噬菌体敏感性、冰核形成、单碳源利用、使用BOXA1R引物的聚合酶链反应（BOX-PCR）以及寄主范围分析的数据表明，导致嫩茎花椰菜叶斑病的病原菌与丁香假单胞菌番茄致病变种相同。青花菜和西兰花的病原菌可感染青花菜、所有测试的十字花科植物、番茄以及三种单子叶植物（加州雀麦、燕麦和普通梯牧草）。其他测试的病原菌（丁香假单胞菌番茄致病变种、丁香假单胞菌番茄致病变种或丁香假单胞菌燕麦致病变种）均未在青花菜上或在十字花科植物和单子叶植物上引起病害。来自噬菌体敏感性、冰核形成、单碳源利用、BOX-PCR以及寄主范围分析的数据表明，青花菜和西兰花的病原菌相同，但与其他测试的致病变种不同，这支持了丁香假单胞菌阿利萨尔致病变种新变种引起西兰花和青花菜叶枯病的假说。
