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本文引用的文献

1
Malaria parasite clag3 genes determine channel-mediated nutrient uptake by infected red blood cells.疟原虫 clag3 基因决定了感染的红细胞通过通道介导的营养摄取。
Cell. 2011 May 27;145(5):665-77. doi: 10.1016/j.cell.2011.05.002.
2
A high-throughput screen targeting malaria transmission stages opens new avenues for drug development.高通量筛选针对疟疾传播阶段为药物开发开辟了新途径。
J Infect Dis. 2011 May 15;203(10):1445-53. doi: 10.1093/infdis/jir037.
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Malaria gametocytogenesis.疟疾配子体生成
Mol Biochem Parasitol. 2010 Aug;172(2):57-65. doi: 10.1016/j.molbiopara.2010.03.019. Epub 2010 Apr 8.
4
Protein export marks the early phase of gametocytogenesis of the human malaria parasite Plasmodium falciparum.蛋白质输出标志着人类疟原虫恶性疟原虫配子体发生的早期阶段。
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Effect of antimalarial drugs on Plasmodium falciparum gametocytes.抗疟药物对恶性疟原虫配子体的作用。
J Infect Dis. 2009 Nov 15;200(10):1518-21. doi: 10.1086/644645.
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Plasmodium falciparum culture: the benefits of shaking.恶性疟原虫培养:振荡培养的益处
Mol Biochem Parasitol. 2010 Jan;169(1):63-5. doi: 10.1016/j.molbiopara.2009.09.005. Epub 2009 Sep 17.
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A green fluorescent protein-based assay for determining gametocyte production in Plasmodium falciparum.一种基于绿色荧光蛋白的用于测定恶性疟原虫配子体产生的检测方法。
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Concentration and purification by magnetic separation of the erythrocytic stages of all human Plasmodium species.通过磁选对所有人类疟原虫物种的红细胞阶段进行浓缩和纯化。
Malar J. 2008 Mar 5;7:45. doi: 10.1186/1475-2875-7-45.
9
Plasmodium falciparum gametocytes: still many secrets of a hidden life.恶性疟原虫配子体:隐秘生活的诸多秘密仍未可知。
Mol Microbiol. 2007 Oct;66(2):291-302. doi: 10.1111/j.1365-2958.2007.05904.x. Epub 2007 Sep 3.
10
Improved synchronous production of Plasmodium falciparum gametocytes in vitro.体外提高恶性疟原虫配子体的同步生产。
Mol Biochem Parasitol. 2007 Jul;154(1):119-23. doi: 10.1016/j.molbiopara.2007.04.008. Epub 2007 Apr 20.

恶性疟原虫配子体的体外培养

Production of Plasmodium falciparum gametocytes in vitro.

作者信息

Saliba Kathryn Shaw, Jacobs-Lorena Marcelo

机构信息

Department of Molecular Microbiology and Immunology, Johns Hopkins University School of Public Health, Baltimore, MD, USA.

出版信息

Methods Mol Biol. 2013;923:17-25. doi: 10.1007/978-1-62703-026-7_2.

DOI:10.1007/978-1-62703-026-7_2
PMID:22990768
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4672730/
Abstract

Production of gametocytes in vitro is essential for studies of Plasmodium falciparum sexual stages. Here, we describe procedures for the high-yield production and fractionation of P. falciparum gametocytes stages I to V.

摘要

体外产生配子体对于恶性疟原虫有性阶段的研究至关重要。在此,我们描述了高产生产和分离恶性疟原虫I至V期配子体的方法。