评估子孢子活力。

Scoring sporozoite motility.

作者信息

Carey Allison F, Ménard Robert, Bargieri Daniel Y

机构信息

Unité de Biologie et Génétique du Paludisme, Institut Pasteur, Paris, France.

出版信息

Methods Mol Biol. 2013;923:371-83. doi: 10.1007/978-1-62703-026-7_26.

DOI:10.1007/978-1-62703-026-7_26

PMID:22990792

Abstract

Sporozoites, the stage of Plasmodium infectious to vertebrates when injected in the skin by a mosquito vector, are highly motile cells. Their unusual form of gliding motility is essential for infectivity, allowing the parasite to travel through both the mosquito and mammalian hosts, invading different cell types and escaping immune cell-mediated death. In this chapter, we describe techniques to study gliding motility of sporozoites in vitro and in vivo.

摘要

子孢子是疟原虫的一个阶段，当被蚊虫载体注入皮肤时，它对脊椎动物具有传染性，是高度能动的细胞。它们独特的滑行运动形式对于感染性至关重要，使寄生虫能够在蚊虫和哺乳动物宿主中穿行，侵入不同的细胞类型并逃避免疫细胞介导的死亡。在本章中，我们描述了在体外和体内研究子孢子滑行运动的技术。