College of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao, 266003, People's Republic of China.
Antonie Van Leeuwenhoek. 2013 Feb;103(2):357-66. doi: 10.1007/s10482-012-9815-y. Epub 2012 Sep 19.
A Gram-negative, strictly aerobic, motile, short rod-shaped bacterium with peritrichous flagella, designated JYr12(T), was isolated from sediment of an amphioxus breeding zone in the coastal region of Qingdao, China. Strain JYr12(T) was found to grow optimally at 28 °C, at pH 7.0 and in the presence of 2-3 % (w/v) NaCl. The organism was determined to contain C(16:0) (28.3 %) and C(16:1) ω7c and/or C(16:1) ω6c (34.8 %) as the major fatty acids and ubiquinone-8 (90 %) as the predominant isoprenoid ubiquinone. The polar lipids were found to consist of phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol and an unknown glycolipid. The G+C content of the genomic DNA was determined to be 42.3 mol %. Comparative 16S rRNA gene sequence analysis revealed that strain JYr12(T) was placed in the class Gammaproteobacteria and shared ~91.20, 91.18, 91.01, 90.95, 90.80 and 90.40 % sequence similarities with representatives of the genus Thalassolituus, Nitrincola, Oceanospirillum, Marinobacterium, Marinomonas and Microbulbifer, respectively. On the basis of phenotypic, chemotaxonomic and genotypic data, strain JYr12(T) is considered to represent a novel species in a new genus within the Gammaproteobacteria, for which the name Litoribacillus peritrichatus gen. nov., sp. nov. is proposed. The type strain of Litoribacillus peritrichatus is JYr12(T) (=CGMCC 1.10796(T) = JCM17551(T)).
一株革兰氏阴性、严格需氧、运动、短杆状细菌，具有周生鞭毛，被命名为JYr12(T)，是从中国青岛沿海地区文昌鱼养殖区的沉积物中分离出来的。菌株 JYr12(T) 被发现最适生长温度为 28°C，最适 pH 值为 7.0，最适盐度为 2-3%（w/v）。该菌含有 C(16:0)（28.3%）和 C(16:1)ω7c 和/或 C(16:1)ω6c（34.8%）作为主要脂肪酸和泛醌-8（90%）作为主要异戊二烯泛醌。极性脂类由磷脂酰甘油、磷脂酰乙醇胺、双磷脂酰甘油和一种未知的糖脂组成。基因组 DNA 的 G+C 含量为 42.3 mol%。比较 16S rRNA 基因序列分析表明，菌株 JYr12(T) 被归入γ变形菌纲，与属 Thalassolituus、Nitrincola、Oceanospirillum、Marinobacterium、Marinomonas 和 Microbulbifer 的代表种的相似性分别为~91.20、91.18、91.01、90.95、90.80 和 90.40%。基于表型、化学分类和基因型数据，菌株 JYr12(T) 被认为是γ变形菌纲中一个新属的新种，建议将其命名为 Litoribacillus peritrichatus gen. nov.，sp. nov.。Litoribacillus peritrichatus 的模式菌株为 JYr12(T)（=CGMCC 1.10796(T)=JCM17551(T)）。
