Laboratório de Virologia Humana, Instituto de Patologia Tropical e Saúde Pública, Goiânia, GO, Brasil.
Mem Inst Oswaldo Cruz. 2012 Sep;107(6):800-4. doi: 10.1590/s0074-02762012000600015.
To determine the positivity rate of human bocavirus (HBoV) 1 and 3 among children who presented with acute gastroenteritis symptoms during the period of 1994-2004 in the Central-West Region of Brazil, 762 faecal samples were tested using polymerase chain reaction (PCR) for the detection of HBoV DNA. Primers for a segment of the non-structural viral protein 1 (NS1) gene of HBoV-1 and HBoV-3 were used. Twelve HBoV-positive samples were further characterised via genomic sequencing and phylogenetic analysis. Of the samples tested, 5.8% (n = 44) were positive for HBoV-1 or HBoV-3 and co-infection was observed in 14 (31.8%) of the 44 HBoV-positive samples. Nine of the 14 samples were also positive for Rotavirus A and five were positive for Aichi virus. The genomic sequencing of the NS1 partial sequence of 12 HBoV-samples showed that 11 samples were characterised as HBoV-1 and that one was characterised as HBoV-3. The phylogenetic analysis showed that the HBoV-1 samples had a high sequence homology to others previously identified in China, Sweden and Brazil. This is the first study conducted in the Central-West Region of Brazil to detect HBoV-1 and HBoV-3 in faecal samples from children with acute gastroenteritis. Further studies are required to define the role of HBoVs as aetiological agents of gastroenteritis.
为了确定 1994 年至 2004 年期间巴西中西部地区出现急性胃肠炎症状的儿童中人博卡病毒（HBoV）1 和 3 的阳性率，使用聚合酶链反应（PCR）检测了 762 份粪便样本以检测 HBoV DNA。使用了针对 HBoV-1 和 HBoV-3 的非结构病毒蛋白 1（NS1）基因片段的引物。对 12 个 HBoV 阳性样本进行了进一步的基因组测序和系统发育分析。在测试的样本中，5.8%（n = 44）对 HBoV-1 或 HBoV-3 呈阳性，在 44 个 HBoV 阳性样本中观察到 14 个（31.8%）合并感染。在 14 个样本中，有 9 个也对轮状病毒 A 呈阳性，有 5 个对 Aichi 病毒呈阳性。12 个 HBoV 样本的 NS1 部分序列的基因组测序表明，11 个样本被确定为 HBoV-1，一个样本被确定为 HBoV-3。系统发育分析显示，HBoV-1 样本与中国、瑞典和巴西以前鉴定的其他样本具有高度的序列同源性。这是巴西中西部地区首次在急性胃肠炎儿童的粪便样本中检测到 HBoV-1 和 HBoV-3。需要进一步的研究来确定 HBoVs 作为胃肠炎病因的作用。
