Sousa Yasmim Gabrielly Souza, Maneschy Carolina Alcântara, Monteiro Carolina Costa, Rodrigues João Victor Souza, Lobo Patrícia Santos, Teixeira Dielle Monteiro, Siqueira Jones Anderson Monteiro, Pinheiro Kenny Costa, Resque Hugo Reis, Silva Luciana Damascena, Guerra Sylvia Fátima Dos Santos, Soares Luana Silva
Postgraduate Program in Virology, Teaching and Scientific Information Section, Evandro Chagas Institute, Secretariat of Health Surveillance and Environment, Brazilian Ministry of Health, Ananindeua 67.030-000, Pará, Brazil.
Laboratory of Gastroenteric Virus, Virology Section, Evandro Chagas Institute, Secretariat of Health Surveillance and Environment, Brazilian Ministry of Health, Ananindeua 67.030-000, Pará, Brazil.
Viruses. 2025 Jan 9;17(1):79. doi: 10.3390/v17010079.
Acute gastroenteritis (AG) is a major illness in early childhood. Recent studies suggest a potential association between human bocavirus (HBoV) and AG. HBoV, a non-enveloped virus with a single-strand DNA genome, belongs to the Parvoviridae family. This study aimed to describe the frequency of HBoV in Northern Brazil using samples from patients with AG collected between 2017 and 2022. Fecal samples obtained from the viral gastroenteritis surveillance network at the Evandro Chagas Institute (IEC) were analyzed. Fecal suspensions (20%) were prepared, and the viral genome was extracted. PCR and nested-PCR were employed to detect HBoV, followed by nucleotide sequencing to identify viral types. Out of 692 samples, HBoV positivity was detected in 9.2% of cases (64/692). Genotypes HBoV-1, HBoV-2, HBoV-3, and HBoV-4 were found in 42.5% (17/40), 22.5% (9/40), 32.5% (13/40), and 2.5% (1/40) of the specimens, respectively. Co-infections with HBoV and other enteric viruses occurred in 48.3% (31/64) of cases, with RVA being the most frequent (31.2%, 20/64). The study results underscore the importance of continuous monitoring and further research to better understand the seasonality, coinfection patterns, and genetic variability of HBoV.
急性肠胃炎（AG）是幼儿期的一种主要疾病。最近的研究表明人博卡病毒（HBoV）与AG之间可能存在关联。HBoV是一种具有单链DNA基因组的无包膜病毒，属于细小病毒科。本研究旨在利用2017年至2022年间收集的AG患者样本，描述巴西北部HBoV的感染频率。对从埃万德罗·查加斯研究所（IEC）的病毒性肠胃炎监测网络获得的粪便样本进行了分析。制备了粪便悬液（20%）并提取了病毒基因组。采用聚合酶链反应（PCR）和巢式PCR检测HBoV，随后进行核苷酸测序以鉴定病毒类型。在692份样本中，9.2%（64/692）的病例检测到HBoV阳性。分别在42.5%（17/40）、22.5%（9/40）、32.5%（13/40）和2.5%（1/40）的标本中发现了HBoV-1、HBoV-2、HBoV-3和HBoV-4基因型。48.3%（31/64）的病例发生了HBoV与其他肠道病毒的共感染，其中轮状病毒A（RVA）最为常见（31.2%，20/64）。研究结果强调了持续监测和进一步研究以更好地了解HBoV的季节性、共感染模式和基因变异性的重要性。
