Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Sekkizhar Campus, Taramani, Chennai 600113, India.
J Cell Biochem. 2013 Mar;114(3):558-69. doi: 10.1002/jcb.24399.
Diethyl hexyl phthalate (DEHP) is a plasticizer, commonly used in a variety of products, including lubricants, perfumes, hairsprays and cosmetics, construction materials, wood finishers, adhesives, floorings and paints. DEHP is an endocrine disruptor and it has a continuum of influence on various organ systems in human beings and experimental animals. However, specific effects of DEHP on insulin signaling in adipose tissue are not known. Adult male albino rats of Wistar strain were divided into four groups. Control, DEHP treated (dissolved in olive oil at a dose of 10, and 100 mg/kg body weight, respectively, once daily through gastric intubations for 30 days) and DEHP + vitamin E (50 mg/kg body weight) and C (100 mg/kg body weight) dissolved in olive oil and distilled water, respectively, once daily through gastric intubations for 30 days. After the completion of treatment, adipose tissue was dissected out to assess various parameters. DEHP treatment escalated H(2)O(2) and hydroxyl radical levels as well as lipid peroxidation in the adipose tissue. DEHP impaired the expression of insulin signaling molecules and their phosphorelay pathways leading to diminish plasma membrane GLUT4 level and thus decreased glucose uptake and oxidation. Blood glucose level was elevated as a result of these changes. Supplementation of vitamins (C & E) prevented the DEHP-induced changes. It is concluded that DEHP-induced ROS and lipid peroxidation disrupts the insulin signal transduction in adipose tissue and favors glucose intolerance. Antioxidant vitamins have a protective role against the adverse effect of DEHP.
邻苯二甲酸二乙酯（DEHP）是一种增塑剂，广泛应用于各种产品，包括润滑剂、香水、发胶和化妆品、建筑材料、木材处理剂、胶粘剂、地板和油漆。DEHP 是一种内分泌干扰物，它对人类和实验动物的各种器官系统都有连续的影响。然而，DEHP 对脂肪组织中胰岛素信号的具体影响尚不清楚。成年雄性 Wistar 白化大鼠分为四组。对照组、DEHP 处理组（分别以 10 和 100mg/kg 体重的剂量溶解在橄榄油中，每日一次通过胃管灌胃 30 天）和 DEHP+维生素 E（50mg/kg 体重）和 C（100mg/kg 体重）溶解在橄榄油和蒸馏水中，每日一次通过胃管灌胃 30 天。治疗完成后，取出脂肪组织评估各种参数。DEHP 处理增加了脂肪组织中的 H(2)O(2)和羟基自由基水平以及脂质过氧化。DEHP 损害了胰岛素信号分子及其磷酸传递途径的表达，导致质膜 GLUT4 水平降低，从而减少葡萄糖摄取和氧化。由于这些变化，血糖水平升高。补充维生素（C 和 E）可预防 DEHP 引起的变化。结论是，DEHP 诱导的 ROS 和脂质过氧化破坏了脂肪组织中的胰岛素信号转导，导致葡萄糖耐量降低。抗氧化维生素对 DEHP 的不良影响具有保护作用。
