Division of Chemistry & Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371.
ACS Nano. 2012 Oct 23;6(10):8546-51. doi: 10.1021/nn301359y. Epub 2012 Sep 25.
Graphene materials are being widely used in electrochemistry due to their versatility and excellent properties as platforms for biosensing. However, no records show the use of inherent redox properties of graphene oxide as a label for detection. Here for the first time we used graphene oxide nanoplatelets (GONPs) as electroactive labels for DNA analysis. The working signal comes from the reduction of the oxygen-containing groups present on the surface of GONPs. The different ability of the graphene oxide nanoplatelets to conjugate to DNA hybrids obtained with complementary, noncomplementary, and one-mismatch sequences allows the discrimination of single-nucleotide polymorphism correlated with Alzheimer's disease. We believe that our findings are very important to open a new route in the use of graphene oxide in electrochemistry.
石墨烯材料因其多功能性和作为生物传感平台的优异性能而在电化学中得到广泛应用。然而，目前没有记录表明氧化石墨烯的固有氧化还原性质可被用作检测标记。在这里，我们首次将氧化石墨烯纳米片（GONPs）用作 DNA 分析的电活性标记物。工作信号来自于 GONPs 表面含氧基团的还原。不同的氧化石墨烯纳米片与互补、非互补和单错配序列获得的 DNA 杂交体的结合能力，使得与阿尔茨海默病相关的单核苷酸多态性的区分成为可能。我们相信，我们的发现对于在电化学中使用氧化石墨烯开辟新途径非常重要。
