Fatema Kamrun Nahar, Liu Yin, Cho Kwang Youn, Oh Won-Chun
Department of Advanced Materials Science & Engineering, Hanseo University, Seosan-si, Chungnam 356-706, South Korea.
College of Materials Science and Engineering, Anhui University of Science & Technology, Huainan 232001, P. R. China.
ACS Omega. 2020 Sep 1;5(36):22719-22730. doi: 10.1021/acsomega.0c00895. eCollection 2020 Sep 15.
Here, we reported an innovative and electrochemical biosensor for the rapid detection of 7. We fabricated the mesoporous ZrO-Ag-G-SiO (ZAGS) and InO-G-SiO (IGS) sensors, and cyclic voltammetry (CV) was employed to detect the bacteria. The development of these portable sensors addresses the challenges of conventional time-consuming and more expensive laboratory-based analyses. Hence, the biosensors were highly selective to detect . The sensor could recognize an individual cell in 1 μL of sample volume within 30 s. live cells tied down on sample nanoparticles worked toward the definite acquirement of . The high thickness of negative charge on the surface of cells effectively regulated the concentration of dominant part charge carriers in the mesoporous channel, allowing a continuous check of concentration in a known sample. The signal current decreased linearly, while the concentration increased from 1.0 × 10 to 1.0 × 10 CFU/mL. ZAGS and IGS biosensors could detect in the range from 10 to 10 CFU/mL. ZAGS and IGS biosensors in this investigation showed great specificity, reproducibility, stability, and selectivity and are expected to have a great impact on applications in the detection of foodborne pathogens.
在此,我们报道了一种用于快速检测7的创新型电化学生物传感器。我们制备了介孔ZrO-Ag-G-SiO(ZAGS)和InO-G-SiO(IGS)传感器,并采用循环伏安法(CV)来检测细菌。这些便携式传感器的开发解决了传统基于实验室的耗时且成本更高的分析所面临的挑战。因此,这些生物传感器对检测具有高度选择性。该传感器能够在30秒内识别1微升样品体积中的单个细胞。固定在样品纳米颗粒上的活细胞有助于明确获取。细胞表面高厚度的负电荷有效地调节了介孔通道中主要部分电荷载流子的浓度,从而能够持续检测已知样品中的浓度。当浓度从1.0×10增加到1.0×10 CFU/mL时,信号电流呈线性下降。ZAGS和IGS生物传感器能够在10至10 CFU/mL的范围内检测。本研究中的ZAGS和IGS生物传感器表现出了极大的特异性、重现性、稳定性和选择性,预计对食源性病原体检测的应用将产生重大影响。