Oehninger Sergio, Morshedi Mahmood, Franken Daniel
The Jones Institute for Reproductive Medicine, Eastern Virginia Medical School, Norfolk, VA, USA.
Methods Mol Biol. 2013;927:91-102. doi: 10.1007/978-1-62703-038-0_9.
The hemizona assay (HZA) has been developed as a diagnostic test for the tight binding of human spermatozoa to the human zona pellucida to predict fertilization potential. In this homologous bioassay, the two matching hemizona halves are functionally equal surfaces allowing controlled comparison of binding from a fertile control versus a test sample, with reproducible measurements of sperm binding obtained from a single oocyte. Oocytes from different sources (surplus from IVF treatment or recovered from ovarian tissue) are salt-stored and used after microbisection. Extensive clinical data have demonstrated excellent predictive power of the HZA for the outcomes of intrauterine insemination and IVF, and therefore the assay has relevance in the clinical diagnostic setting in infertility.
半透明带分析（HZA）已被开发出来，作为一种诊断测试，用于检测人类精子与人类透明带的紧密结合，以预测受精潜力。在这种同源生物测定中，两个匹配的半透明带半部是功能相等的表面，允许对来自可育对照与测试样品的结合进行可控比较，并从单个卵母细胞获得可重复的精子结合测量值。来自不同来源（体外受精治疗后的多余卵子或从卵巢组织中回收的卵子）的卵母细胞经盐储存后，在显微切割后使用。大量临床数据表明，HZA对宫内人工授精和体外受精的结果具有出色的预测能力，因此该分析在不孕症的临床诊断环境中具有相关性。
