Kruger T F, Oehninger S, Franken D R, Hodgen G D
Tygerberg Hospital, Department of Obstetrics and Gynaecology, Republic of South Africa.
J In Vitro Fert Embryo Transf. 1991 Jun;8(3):154-6. doi: 10.1007/BF01131706.
The hemizona assay (HZA) has a high predictive value for in vitro fertilization (IVF) results. Oocyte quality plays a significant role in the validation of this test. The question was asked whether human salt-stored oocytes (up to 30 days) are damaged and subsequently lose their sperm binding capacity when compared to fresh human oocytes. Equivalent binding in both the salt-stored and the fresh group of oocytes was observed in the hemizonae incubated with normal semen as well as in their matching halves incubated with semen from an infertile man. Based on the results, we conclude that salt-stored oocytes (pH 7.0) give reliable information regarding sperm binding potential under HZA conditions.
半透明带分析（HZA）对体外受精（IVF）结果具有较高的预测价值。卵母细胞质量在该检测的验证中起着重要作用。有人提出一个问题，即与新鲜人类卵母细胞相比，人类盐储存卵母细胞（长达30天）是否会受损并随后丧失其精子结合能力。在用正常精液孵育的半透明带以及用不育男性精液孵育的配对半透明带中，观察到盐储存卵母细胞组和新鲜卵母细胞组的结合情况相当。基于这些结果，我们得出结论，盐储存卵母细胞（pH 7.0）在HZA条件下能提供有关精子结合潜力的可靠信息。
