Kashou Anthony H, Sharma Rakesh, Agarwal Ashok
Center for Reproductive Medicine, Glickman Urological & Kidney Institute and Ob/Gyn & Women's Health Institute, Cleveland Clinic, Cleveland, OH, USA.
Methods Mol Biol. 2013;927:351-61. doi: 10.1007/978-1-62703-038-0_30.
The chemiluminescence method is the most commonly employed technique as a direct measurement of reactive oxygen species (ROS) generation by spermatozoa. This assay is capable of quantifying both intracellular and extracellular ROS. Moreover, the use of various probes allows for differentiation between superoxide and hydrogen peroxide production by spermatozoa. When the total antioxidant reserves are overwhelmed by excessive production of ROS, it results in oxidative stress. Therefore correct measurement of both ROS and total antioxidant capacity (TAC) is essential in the assessment of oxidative stress in sperm and semen. This chapter describes the methodological approach for measuring seminal oxidative stress through the use of chemiluminescence assay for accurate measurement of ROS and the colorimetric assay for measurement of TAC.
化学发光法是最常用的技术，用于直接测量精子产生的活性氧（ROS）。该检测方法能够定量细胞内和细胞外的ROS。此外，使用各种探针可以区分精子产生的超氧化物和过氧化氢。当总抗氧化储备被过量产生的ROS overwhelm时，就会导致氧化应激。因此，正确测量ROS和总抗氧化能力（TAC）对于评估精子和精液中的氧化应激至关重要。本章介绍了通过使用化学发光法准确测量ROS和比色法测量TAC来测量精液氧化应激的方法。 （注：原文中“overwhelm”此处翻译为“压倒、超过负荷”不太准确，结合语境似乎是指抗氧化储备被ROS大量产生所“打破平衡”等意思，但按要求不添加解释，直接翻译为“压倒”。）
