Experimental and Clinical Physiopathology Research Group, Department of Health Sciences, School of Experimental Sciences, University of Jaen, Jaen, Spain.
Anticancer Res. 2012 Sep;32(9):3675-82.
Angiotensin peptides play roles in brain tumor infiltration and associated angiogenesis.
We explored the roles of soluble and membrane-bound forms of renin-angiotensin system-regulating aminopeptidase N (APN)-, aminopeptidase B (APB)-, glutamate aminopeptidase- and aspartate aminopeptidase (AspAP)-specific activities on tumor growth in the rat C6 glioma model with implantation into the subcutaneous abdomen of Wistar rats, evaluating these activities as biological markers. The tumor volume was assessed for three weeks and a sample of tumor was obtained every seven days to obtain the soluble and membrane-bound fraction, in order to assay enzyme activities fluorometrically using their corresponding aminoacyl-β-naphthylamide as substrates.
We found a time-dependent decrease in soluble and membrane-bound APN and APB. Soluble AspAP increases with tumor growth in a time-dependent manner.
Although gliomas are heterogeneous tissues, angiotensin peptides are involved in this model of tumor growth and their role could be analyzed through their corresponding regulatory proteolytic enzymes.
血管紧张肽在脑肿瘤浸润和相关血管生成中发挥作用。
我们探索了可溶性和膜结合形式的肾素-血管紧张素系统调节氨基肽酶 N（APN）、氨基肽酶 B（APB）、谷氨酸氨基肽酶和天冬氨酸氨基肽酶（AspAP）特异性活性在大鼠 C6 神经胶质瘤模型中的肿瘤生长中的作用，将这些活性作为生物标志物进行评估。肿瘤体积在三周内进行评估，每隔七天取一次肿瘤样本，以获得可溶性和膜结合部分，并用相应的氨基酸酰基-β-萘酰胺作为底物通过荧光法测定酶活性。
我们发现可溶性和膜结合的 APN 和 APB 随时间呈下降趋势。可溶性 AspAP 随肿瘤生长呈时间依赖性增加。
尽管神经胶质瘤是异质性组织，但血管紧张肽参与了这种肿瘤生长模型，它们的作用可以通过其相应的调节蛋白水解酶进行分析。
