Han Kyoung-Hee, Kim Sung-Hoon, Jeong In Cheol, Lee Young-Hee, Chang Sei-Jin, Park Bit-Na-Ri, Kim Seok Won
Department of Obstetrics and Gynecology, Yonsei University Wonju College of Medicine, Wonju, Korea.
J Korean Neurosurg Soc. 2012 Jul;52(1):32-6. doi: 10.3340/jkns.2012.52.1.32. Epub 2012 Jul 31.
Alcoholic neuropathy is characterized by allodynia (a discomfort evoked by normally innocuous stimuli), hyperalgesia (an exaggerated pain in response to painful stimuli) and spontaneous burning pain. The aim of the present study is to investigate the effect of rolipram, a phosphodiesterase 4 inhibitor, against alcohol-induced neuropathy in rats.
Allodynia was induced by administering 35% v/v ethanol (10 g/kg; oral gavage) to Spraue-Dawley rats for 8 weeks. Rolipram and saline (vehicle) were administered intraperitoneally. Mechanical allodynia was measured by using von Frey filaments. Somatosensory evoked potential (SEP) was proposed as complementary measure to assess the integrity of nerve pathway.
The ethanol-induced mechanical allodynia began to manifest from 3 week, and then peaked within 1 week. Beginning from 3 week, latency significantly started to increased in control group. In rolipram treated rats, the shorter latency was sustained until 8 weeks (p<0.05). The mechanical allodynia, which began to manifest on the 3 weeks, intraperitoneal injections of rolipram sustained statistical difference until 8 weeks, the final week of the study (p<0.05).
This study suggests that rolipram might alleviate mechanical allodynia induced by alcohol in rats, which clearly has clinical implication.
酒精性神经病变的特征为痛觉过敏（由通常无害的刺激所诱发的不适）、痛觉超敏（对疼痛刺激的过度疼痛反应）和自发性灼痛。本研究的目的是调查磷酸二酯酶4抑制剂咯利普兰对大鼠酒精诱导性神经病变的影响。
通过对Spraue-Dawley大鼠灌胃给予35% v/v乙醇（10 g/kg）8周来诱导痛觉过敏。咯利普兰和生理盐水（赋形剂）通过腹腔注射给药。使用von Frey细丝测量机械性痛觉过敏。体感诱发电位（SEP）被用作评估神经通路完整性的补充指标。
乙醇诱导的机械性痛觉过敏在第3周开始显现，然后在1周内达到峰值。从第3周开始，对照组的潜伏期显著开始增加。在咯利普兰治疗的大鼠中，较短的潜伏期持续到8周（p<0.05）。在第3周开始显现的机械性痛觉过敏，腹腔注射咯利普兰直到研究的最后一周即第8周都保持统计学差异（p<0.05）。
本研究表明咯利普兰可能减轻大鼠酒精诱导的机械性痛觉过敏，这显然具有临床意义。
