Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, USA.
J Am Chem Soc. 2012 Oct 3;134(39):16099-102. doi: 10.1021/ja304373u. Epub 2012 Sep 20.
A molecular probe for the luminescent detection of adenosine nucleotides is presented. The probe, Tb-DOTAm-Phen, readily distinguishes among the three adenosine nucleotides in buffered aqueous conditions at neutral pH, a requirement for the direct monitoring of enzymatic reactions converting adenosine triphosphate (ATP) to adenosine diphosphate or adenosine monophosphate. The probe is most efficient under millimolar concentrations of ATP which are relevant to intracellular conditions. Moreover, the long luminescence lifetime of the probe readily enables time-gating experiments.
本文介绍了一种用于检测腺苷核苷酸的分子探针。该探针 Tb-DOTAm-Phen 在中性 pH 缓冲水溶液中可轻松区分三种腺苷核苷酸，这是直接监测将三磷酸腺苷 (ATP) 转化为二磷酸腺苷或一磷酸腺苷的酶反应所必需的条件。在与细胞内条件相关的毫摩尔浓度的 ATP 下，该探针的效率最高。此外，该探针的长荧光寿命使其能够轻松进行时间门控实验。
