Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Erandwane, Pune, Maharashtra, India.
Pharm Biol. 2012 Nov;50(11):1428-35. doi: 10.3109/13880209.2012.684064. Epub 2012 Sep 21.
Lagenaria siceraria (Molina) Standl. (Cucurbitacae) (LS) has been reported to possess cardioprotective, antihyperlipidemic, and diuretic activities.
To evaluate antihypertensive and cardioprotective effects of the Lagenaria siceraria fruit powder in N(G)-nitro-L-arginine methyl ester (L-NAME) induced hypertension in rats.
Male Wistar rats were divided in four groups. Control 2% gum acacia p.o., L-NAME (40 mg/kg p.o.), LS (500 mg/kg p.o.) + L-NAME (40 mg/kg p.o.), L-arginine (100 mg/kg p.o.) + L-NAME (40 mg/kg p.o.). Treatment period was 4 weeks. On day 29 serum marker enzymes, cholesterol and heamodynamic parameters were measured. Histology of heart was performed. LS powder was characterized by HPLC.
Systolic blood pressures were increased by L-NAME (p < 0.001). In both drug treated groups systolic and diastolic blood pressures were reduced significantly (p < 0.001) compared to L-NAME. In L-NAME group significantly (p < 0.01) elevated cholesterol which was reduced (p < 0.05) by LS treatment. In L-NAME group inflammation and necrosis (0-35%) was present in heart whereas there was no change in myocardium of LS and L-arginine treated rats. Vitexin, orientin and isoorientin were detected in methanol extract of LS powder.
L-NAME induced hypertension in rats was reduced by treatment with LS. The absence of necrosis, inflammation in the heart and significant reduction in serum cholesterol in LS and L-arginine treated rats indicated cardioprotective activity. Antioxidant activity of orientin and isoorientin appears to reduce the L-NAME induced damage. It is concluded that LS fruit possess antihypertensive and cardioprotective activity.
葫芦科（Cucurbitaceae）苦瓜（Molina）Standl.（LS）已被报道具有心脏保护、抗高血脂和利尿作用。
评价苦瓜果粉对 N(G)-硝基-L-精氨酸甲酯（L-NAME）诱导的高血压大鼠的降压和心脏保护作用。
雄性 Wistar 大鼠分为 4 组。对照组 2%胶阿拉伯树胶口服，L-NAME（40mg/kg 口服），LS（500mg/kg 口服）+ L-NAME（40mg/kg 口服），L-精氨酸（100mg/kg 口服）+ L-NAME（40mg/kg 口服）。治疗期为 4 周。第 29 天测量血清标志物、胆固醇和血液动力学参数。进行心脏组织学检查。LS 粉末用 HPLC 进行特征描述。
L-NAME 使收缩压升高（p < 0.001）。与 L-NAME 相比，两种药物治疗组的收缩压和舒张压均显著降低（p < 0.001）。在 L-NAME 组中，胆固醇显著升高（p < 0.01），而 LS 治疗则降低（p < 0.05）。在 L-NAME 组中，心脏有炎症和坏死（0-35%），而 LS 和 L-精氨酸治疗大鼠的心肌没有变化。在 LS 粉末的甲醇提取物中检测到牡荆素、荭草苷和异荭草苷。
LS 治疗可减轻 L-NAME 诱导的高血压大鼠的高血压。LS 和 L-精氨酸治疗大鼠的心脏无坏死、炎症，血清胆固醇显著降低，表明具有心脏保护作用。荭草苷和异荭草苷的抗氧化活性似乎减轻了 L-NAME 诱导的损伤。结论是 LS 果实具有降压和心脏保护作用。
