Hassan Zeinab Korany, Daghestani Maha Hassan
Department of Zoology, Center for Scientific and Medical Female Colleges, King Saud University, Riyadh, Saudi Arabia.
Asian Pac J Cancer Prev. 2012;13(7):3259-64. doi: 10.7314/apjcp.2012.13.7.3259.
Curcumin (CM) possesses anti-cancer activity against a variety of tumors. Matrix metalloproteinases (MMPs) play an important role in remodeling the extracellular matrix and their activities are regulated by tissue inhibitor of metalloproteinases (TIMPs) family. Control of MMP and TIMP activity are now of great significance. In this study, the effect of CM is investigated on metastatic MMPs and anti-metastatic TIMPs genes on MDA breast cancer cells cultured in a mixture of DMEM and Ham's F12 medium and treated with different concentrations of CM (10, 20 and 40 μM for various lengths of time. Reverse transcription followed by quantitative real time PCR was used to detect the gene expression levels of MMPs and TIMPs in CM-treated versus untreated cases and the data were analyzed by one-way ANOVA. At high concentrations of curcumin, TIMP-1, -2, -3 and -4 genes were up-regulated after 48 hours of treatment, their over-expression being accompanied by down-regulation of MMP-2 and MMP-9 gene expression levels in a concentration- and time-dependent manner. These results suggest that curcumin plays a role in regulating cell metastasis by inhibiting MMP-2 and MMP-9 and up-regulating TIMP1 and TIMP4 gene expression in breast cancer cells.
姜黄素（CM）对多种肿瘤具有抗癌活性。基质金属蛋白酶（MMPs）在细胞外基质重塑中起重要作用，其活性受金属蛋白酶组织抑制剂（TIMPs）家族调控。控制MMP和TIMP的活性目前具有重要意义。在本研究中，研究了CM对在DMEM和Ham's F12培养基混合物中培养并用不同浓度CM（10、20和40μM，处理不同时长）处理的MDA乳腺癌细胞中转移性MMPs和抗转移性TIMPs基因的影响。采用逆转录后进行定量实时PCR来检测CM处理组与未处理组中MMPs和TIMPs的基因表达水平，并通过单因素方差分析对数据进行分析。在高浓度姜黄素处理48小时后，TIMP - 1、-2、-3和-4基因上调，其过表达伴随着MMP - 2和MMP - 9基因表达水平以浓度和时间依赖性方式下调。这些结果表明，姜黄素通过抑制乳腺癌细胞中的MMP - 2和MMP - 9以及上调TIMP1和TIMP4基因表达，在调节细胞转移中发挥作用。
