谷胱甘肽-S-转移酶基因多态性在通过吸烟和幽门螺杆菌感染状况预测胃癌风险中的作用

Glulathione-S-transferases gene polymorphism in prediction of gastric cancer risk by smoking and Helicobacter pylori infection status.

作者信息

Jing Chen, Huang Zhi-Jie, Duan Yu-Qin, Wang Pei-Hong, Zhang Ru, Luo Ke-Shu, Xiao Xin-Rong

机构信息

Department of Cadre Ward, the General Hospital of Chengdu Military Area, Chengdu, China.

出版信息

Asian Pac J Cancer Prev. 2012;13(7):3325-8. doi: 10.7314/apjcp.2012.13.7.3325.

DOI:10.7314/apjcp.2012.13.7.3325

PMID:22994755

Abstract

AIM

To evaluate the association of glutathione S-transferases gene polymorphisms with the risk of gastric cancer, with reference to smoking and Helicobacter pylori infection.

METHODS

We conducted a 1:1 matched case-control study with 410 gastric cancer cases and 410 cancer-free controls. Polymorphisms of GSTM1, GSTT1 and GSTP1 were determined using PCR-CTPP.

RESULTS

The GSTM1 and GSTT1 null genotypes were significantly associated with the risk of gastric cancer after adjusting for potential confounding factors (OR=1.68, 95% CI=1.32-2.23 for null GSTM1, OR=1.73; 95% CI=1.24-2.13 for null GSTT1). The combination of null GSTM1 and null GSTT1 conferred an elevated risk (OR=2.54, 95% CI=1.55-3.39). However, no association was found for GSTP1 polymorphism The smoking modified the association of GSTM1 and GSTT1 null genotypes with the risk of gastric cancer.

CONCLUSION

GSTM1 and GSTT1 null genotypes are associated with increased risk of gastric cancer, and smoking modifies the association.

摘要

目的

参照吸烟和幽门螺杆菌感染情况，评估谷胱甘肽S-转移酶基因多态性与胃癌风险的相关性。

方法

我们开展了一项1:1匹配的病例对照研究，纳入410例胃癌病例和410例无癌对照。采用聚合酶链反应-互补脱氧核糖核酸引物延伸预扩增法测定GSTM1、GSTT1和GSTP1的多态性。

结果

在对潜在混杂因素进行校正后，GSTM1和GSTT1无效基因型与胃癌风险显著相关（GSTM1无效基因型的比值比=1.68，95%置信区间=1.32-2.23；GSTT1无效基因型的比值比=1.73，95%置信区间=1.24-2.13）。GSTM1无效基因型和GSTT1无效基因型共同存在时风险升高（比值比=2.54，95%置信区间=1.55-3.39）。然而，未发现GSTP1多态性与胃癌风险存在关联。吸烟改变了GSTM1和GSTT1无效基因型与胃癌风险的相关性。