Biology Institute, Hebei Academy of Sciences, Shijiazhuang 050051, China.
Biochem Biophys Res Commun. 2012 Oct 19;427(2):293-8. doi: 10.1016/j.bbrc.2012.09.044. Epub 2012 Sep 17.
N-acyl-homoserine lactones (AHLs) are a class of bacterial quorum-sensing (QS) signals that are commonly used by Gram-negative bacteria for cell-to-cell communication. Recently, it has become evident that AHLs can regulate plant root growth and trigger plant defense responses; however, little is known about the plant response mechanisms to bacterial QS signals. In this study, we used a proteomic approach to investigate the responses of Arabidopsis thaliana seedlings to N-3-oxo-octanoyl-homoserine lactone (3OC8-HSL), a bacterial QS signal. The results revealed that the abundance of 53 protein spots was significantly altered; two thirds of these proteins were found to be up-regulated after 3OC8-HSL treatment. Thirty-four proteins were identified using MALDI-TOF-MS. These 3OC8-HSL-responsive proteins, in addition to one protein of unknown function, are implicated in a variety of physiological processes, including metabolism of carbohydrate and energy, protein biosynthesis and quality control systems, defense response and signal transduction and cytoskeleton remodeling. Our bioinformatic analysis indicated that the chloroplasts are the intracellular organelles most influenced by the exposure to 3OC8-HSL. Our data indicate that plants have an extensive range of functional responses to bacterial AHLs that may play important roles in the interaction between plants and bacteria.
N-酰基高丝氨酸内酯（AHLs）是一类细菌群体感应（QS）信号分子，通常被革兰氏阴性细菌用于细胞间通讯。最近，人们已经意识到 AHLs 可以调节植物根系生长并触发植物防御反应；然而，对于植物对细菌 QS 信号的反应机制知之甚少。在这项研究中，我们使用蛋白质组学方法研究了拟南芥幼苗对 N-3-氧代-辛酰高丝氨酸内酯（3OC8-HSL）的反应，3OC8-HSL 是一种细菌 QS 信号分子。结果表明，53 个蛋白点的丰度明显改变；其中三分之二的蛋白在 3OC8-HSL 处理后上调。使用 MALDI-TOF-MS 鉴定了 34 种蛋白质。这些 3OC8-HSL 反应蛋白，除了一种功能未知的蛋白质外，参与了多种生理过程，包括碳水化合物和能量代谢、蛋白质生物合成和质量控制系统、防御反应和信号转导以及细胞骨架重塑。我们的生物信息学分析表明，暴露于 3OC8-HSL 后，叶绿体是受影响最大的细胞内细胞器。我们的数据表明，植物对细菌 AHLs 有广泛的功能反应，这可能在植物与细菌的相互作用中发挥重要作用。
