Department of Biomaterials, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawara-cho Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
Acta Biomater. 2013 Feb;9(2):5194-200. doi: 10.1016/j.actbio.2012.07.039. Epub 2012 Sep 17.
ProNectin F (PnF) was chemically modified by introducing some functional groups to prepare various derivatives of primary amino (PnF-N₁), tertiary amino (PnF-N₃), quaternary ammonium (PnF-N₄), carboxyl (PnF-COOH) and sulfonyl groups (PnF-SO₃H). When C3H10T1/2 cells were cultured on non-treated dishes coated with the derivatives, the number of mesenchymal cells attached to the culture dishes increased for the coating with PnF-COOH and PnF-SO₃H, even at their low adsorption amount. The cytotoxicity was high for the coating of PnF-N₁ and PnF-N₄ compared with that of the PnF-N₃, PnF-COOH and PnF-SO₃H. The treatment with integrin α5 and αV antibodies suppressed the cell attachment to the dishes coated with PnF-COOH and PnF-SO₃H. The phosphorylation of extracellular signal-regulated kinase (ERK) was upregulated for cells attached to the dishes coated with PnF-COOH and PnF-SO₃H, indicating their enhanced proliferation. It is concluded that the chemical derivatization of PnF enhanced the ability of cell attachment and proliferation.
ProNectin F（PnF）通过引入一些功能基团进行化学修饰，制备了各种伯氨基（PnF-N₁）、叔氨基（PnF-N₃）、季铵盐（PnF-N₄）、羧基（PnF-COOH）和砜基（PnF-SO₃H）衍生物。当 C3H10T1/2 细胞在未处理的培养皿上培养时，用 PnF-COOH 和 PnF-SO₃H 包被的培养皿上黏附的间充质细胞数量增加，即使它们的吸附量很低。与 PnF-N₃、PnF-COOH 和 PnF-SO₃H 相比，PnF-N₁ 和 PnF-N₄ 的涂层具有更高的细胞毒性。整合素 α5 和 αV 抗体的处理抑制了细胞黏附到用 PnF-COOH 和 PnF-SO₃H 包被的培养皿上。黏附到用 PnF-COOH 和 PnF-SO₃H 包被的培养皿上的细胞中细胞外信号调节激酶（ERK）的磷酸化被上调，表明其增殖增强。综上所述，PnF 的化学衍生化增强了细胞黏附和增殖的能力。
