Kreutzer R D, Palau M T, Morales A, Ferro C, Feliciangeli D, Young D G
Biology Department, Youngstown State University, Ohio 44555.
J Med Entomol. 1990 Jan;27(1):1-8. doi: 10.1093/jmedent/27.1.1.
Isozyme data were used to identify populations of certain Lutzomyia taxa in the verrucarum species group, mostly in the series townsendi. Lutzomyia youngi Feliciangelis and Murillo and L. spinicrassa Morales, Osorno, Osorno, and Hoyos each have diagnostic allomorphs for phosphogluconate dehydrogenase (6PGDH) and fumarate hydratase (FUM). The 6PGDH and FUM data and those from 6-phospho-fructokinase and phosphoglucomutase distinguish Lutzomyia sp., a new species from Columbia to be described and named later. Data from these enzymes and glucose phosphate isomerase will separate L. townsendi (Ortiz) from the others and from L. longiflocosa Osorno, Morales, Osorno, and Hoyos, L. quasitownsendi Morales, Osorno, Osorno, and Hoyos, and L. sauroida Osorno, Morales, and Osorno (three species that are inseparable using enzyme data). Three other species, L. serrana (Damasceno and Arouck) (series serrana) and L. columbiana (Ristorcelli and Van Ty) and L. andina Osorno, Osorno, and Morales (both in series verrucarum), are morphologically distinct using conventional characters and have fixed diagnostic differences at several enzyme loci. Statistical analyses of the enzyme data using genetic identities (I), differences (D), and the amount of genetic variation among these taxa indicated that such statistics can be as useful in the study of sand fly phylogeny and population genetics as they have been for other organisms. I and D values indicated that L. longiflocosa, L. quasitownsendi, and L. sauroida are very similar (I = 0.991 and D = 0.010) and possibly are populations of the same conspecific species. The levels of divergence, based on combined enzyme data for up to 21 gene loci among the taxa, are discussed, and a dendrogram based on genetic distance is presented. The genetic data confirmed established phylogenetic relationships among the sand fly taxa based on structural similarities.
同工酶数据被用于鉴定疣蚤蝇物种组中某些卢氏蚤蝇类群的种群，主要是汤森氏系列。扬氏卢氏蚤蝇（费利西安热利斯和穆里罗）以及刺粗卢氏蚤蝇（莫拉莱斯、奥索诺、奥索诺和霍约斯）在磷酸葡萄糖酸脱氢酶（6PGDH）和延胡索酸水合酶（FUM）方面均具有诊断性的同种异型。6PGDH和FUM数据以及来自6-磷酸果糖激酶和磷酸葡萄糖变位酶的数据可区分一种来自哥伦比亚的新物种卢氏蚤蝇，该物种稍后将被描述和命名。这些酶和磷酸葡萄糖异构酶的数据将汤森氏卢氏蚤蝇（奥尔蒂斯）与其他物种以及长鬃卢氏蚤蝇（奥索诺、莫拉莱斯、奥索诺和霍约斯）、拟汤森氏卢氏蚤蝇（莫拉莱斯、奥索诺、奥索诺和霍约斯）和蜥形卢氏蚤蝇（奥索诺、莫拉莱斯和奥索诺）（这三个物种利用酶数据无法区分）区分开来。另外三个物种，锯齿卢氏蚤蝇（达马塞诺和阿鲁克）（锯齿系列）、哥伦比亚卢氏蚤蝇（里斯托塞利和范蒂）以及安第斯卢氏蚤蝇（奥索诺、奥索诺和莫拉莱斯）（均在疣蚤蝇系列），利用传统特征在形态上有明显差异，并且在几个酶位点上有固定的诊断性差异。使用遗传同一性（I）、差异（D）以及这些类群之间的遗传变异量对酶数据进行的统计分析表明，此类统计在白蛉系统发育和种群遗传学研究中与在其他生物研究中一样有用。I和D值表明长鬃卢氏蚤蝇、拟汤森氏卢氏蚤蝇和蜥形卢氏蚤蝇非常相似（I = 0.991，D = 0.010），可能是同一物种的种群。基于多达21个基因位点的组合酶数据讨论了这些类群之间的分歧水平，并给出了基于遗传距离的树状图。遗传数据证实了基于结构相似性的白蛉类群之间已确立的系统发育关系。
