Arthropod-Borne Animal Disease Research Unit, Center for Grain and Animal Health Research, Agricultural Research Service, United States Department of Agriculture, 1515 College Avenue, Manhattan, KS 66502, USA.
Am J Trop Med Hyg. 2011 Jun;84(6):913-22. doi: 10.4269/ajtmh.2011.11-0040.
Within the sand fly genus Lutzomyia, the Verrucarum species group contains several of the principal vectors of American cutaneous leishmaniasis and human bartonellosis in the Andean region of South America. The group encompasses 40 species for which the taxonomic status, phylogenetic relationships, and role of each species in disease transmission remain unresolved. Mitochondrial cytochrome c oxidase I (COI) phylogenetic analysis of a 667-bp fragment supported the morphological classification of the Verrucarum group into series. Genetic sequences from seven species were grouped in well-supported monophyletic lineages. Four species, however, clustered in two paraphyletic lineages that indicate conspecificity--the Lutzomyia longiflocosa-Lutzomyia sauroida pair and the Lutzomyia quasitownsendi-Lutzomyia torvida pair. COI sequences were also evaluated as a taxonomic tool based on interspecific genetic variability within the Verrucarum group and the intraspecific variability of one of its members, Lutzomyia verrucarum, across its known distribution.
在沙蝇属的 Lutzomyia 中，Verrucarum 物种群包含了南美洲安第斯地区几种主要的美洲皮肤利什曼病和人类巴尔通体病的病媒。该群包括 40 种，其分类地位、系统发育关系以及每种在疾病传播中的作用仍未解决。对 667bp 片段的线粒体细胞色素 c 氧化酶 I (COI) 系统发育分析支持将 Verrucarum 群分为系列的形态分类。来自七个物种的遗传序列分为支持良好的单系。然而，有四个物种聚类在两个非单系的谱系中，表明它们是同一种群——Lutzomyia longiflocosa-Lutzomyia sauroida 对和 Lutzomyia quasitownsendi-Lutzomyia torvida 对。还根据 Verrucarum 群内种间遗传变异性以及其成员之一 Lutzomyia verrucarum 在其已知分布范围内的种内变异性，将 COI 序列评估为一种分类工具。
