Department of Chinese Cabbage, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Theor Appl Genet. 2013 Jan;126(1):179-88. doi: 10.1007/s00122-012-1972-x. Epub 2012 Sep 21.
The extreme resistance to Turnip mosaic virus observed in the Chinese cabbage (Brassica rapa) line, BP8407, is monogenic and recessive. Bulked segregant analysis was carried out to identify simple sequence repeat and Indel markers linked to this recessive resistance gene, termed recessive Turnip mosaic virus resistance 02 (retr02). Mapping of PCR-specific Indel markers on 239 individuals of a BP8407 × Ji Zao Chun F(2) population, located this resistance gene to a 0.9-cM interval between two Indel markers (BrID10694 and BrID101309) and in scaffold000060 or scaffold000104 on chromosome A04 of the B. rapa genome. Eleven eukaryotic initiation factor 4E (eIF4E) and 14 eukaryotic initiation factor 4G (eIF4G) genes are predicted in the B. rapa genome. A candidate gene, Bra035393 on scaffold000104, was predicted within the mapped resistance locus. The gene encodes the eIF(iso)4E protein. Bra035393 was sequenced in BP8407 and Ji Zao Chun. A polymorphism (A/G) was found in exon 3 between BP8407 and Ji Zao Chun. This gene was analysed in four resistant and three susceptible lines. A correlation was observed between the amino acid substitution (Gly/Asp) in the eIF(iso)4E protein and resistance/susceptibility. eIF(iso)4E has been shown previously to interact with the TuMV genome-linked protein, VPg.
白菜（Brassica rapa）品系 BP8407 对芜菁花叶病毒表现出极强的抗性，该抗性为单基因隐性遗传。利用混池分离分析法，鉴定到与该隐性抗病基因连锁的简单重复序列和插入缺失标记，将其命名为隐性芜菁花叶病毒抗性 02（retr02）。将 BP8407×冀早春 F2 群体 239 个个体上的 PCR 特异性 Indel 标记进行定位，将该抗病基因定位在两个 Indel 标记（BrID10694 和 BrID101309）之间的 0.9-cM 区间内，位于白菜 A04 染色体的 scaffold000060 或 scaffold000104 上。在白菜基因组中预测到 11 个真核起始因子 4E（eIF4E）和 14 个真核起始因子 4G（eIF4G）基因。在定位的抗病基因座内预测到候选基因 scaffold000104 上的 Bra035393。该基因编码 eIF（iso）4E 蛋白。在 BP8407 和冀早春中对 Bra035393 进行了测序。在 BP8407 和冀早春之间发现了外显子 3 中的多态性（A/G）。该基因在 4 个抗性和 3 个感病品系中进行了分析。观察到 eIF（iso）4E 蛋白中的氨基酸替换（Gly/Asp）与抗性/敏感性之间存在相关性。eIF（iso）4E 先前已被证明与 TuMV 基因组连接蛋白 VPg 相互作用。
