VALWAY Technology Center, NEC Soft Ltd., Tokyo, Japan.
Proteomics Clin Appl. 2012 Dec;6(11-12):574-80. doi: 10.1002/prca.201200037. Epub 2012 Nov 8.
Aptamers are functional oligonucleotides of single-stranded RNA or DNA that can selectively recognize their targets with high affinity. Hence, they have been widely developed for analytical, diagnostic, and therapeutic applications. In this review, we have summarized recent advances in the development of aptamer-based detection systems. Aptamers can be amplified exponentially by PCR, which is one of the advantages of aptamers over antibodies. Recently, we have developed immuno-aptamers that bind to mouse or rabbit IgG and constructed a novel sensitive detection system based on a conventional ELISA, called the immuno-aptamer PCR assay. In this article, the aptamer-based ready-to-use sensors and another PCR-based aptamer assays are also described; moreover, we have discussed highly sensitive aptamer-based detection systems.
适体是单链 RNA 或 DNA 的功能性寡核苷酸，能够高亲和力选择性地识别其靶标。因此，它们已被广泛开发用于分析、诊断和治疗应用。在这篇综述中，我们总结了基于适体的检测系统的最新进展。适体可以通过 PCR 指数扩增，这是适体优于抗体的优势之一。最近，我们开发了与小鼠或兔 IgG 结合的免疫适体，并基于常规 ELISA 构建了一种新的敏感检测系统，称为免疫适体 PCR 测定法。在本文中，还描述了基于适体的即用型传感器和另一种基于 PCR 的适体测定法；此外，我们还讨论了基于适体的高灵敏度检测系统。
