Dipartimento di Medicina ed Oncologia Sperimentale, Università di Torino, Corso Raffello 30, 10125, Turin, Italy.
Hernia. 2013 Apr;17(2):241-8. doi: 10.1007/s10029-012-0996-0. Epub 2012 Sep 21.
Abdominal wall hernia is one of the commonest surgical disorders worldwide, and there is no single gold-standard operative technique to repair it. In an effort to improve techniques and technologies to reinforce hernia repair, synthetic meshes are employed. In this study, a new prosthesis (named composite) formed of two polypropylene layers, one macroporous (named mesh) and one transparent (named film), was examined to evaluate its capability to enable cell proliferation without inducing cell death. Inflammatory processes were also examined.
Human fibroblasts BJ were seeded on multiwells, on which composite or film had been placed. After 7, 14, and 21 days, cell growth and viability, deposition of collagen, and release of IL-6, IL-1β, and TNF-α were evaluated.
The "in vitro" protocol showed the composite to be colonized by human fibroblasts on the polypropylene macroporous mesh side; no cell growth occurred on the film. The slowdown of cell growth observed between 14 and 21 days was accompanied by an increase in type I collagen deposition and marked fibroblast activity. Inflammatory cytokines initially increased, followed by their reduction beginning at 14 days.
The new prosthesis comprising two polypropylene layers of differing morphologies can be colonized by fibroblasts on the side facing the abdominal wall, whereas no cell growth occurs on the side facing the viscera. The transient inflammation, observed at early experimental times, is probably important for the healing process.
腹壁疝是全球最常见的外科疾病之一，目前尚无单一的金标准手术技术来修复它。为了改进加强疝修补技术和技术，合成网片被应用。在这项研究中，我们检查了一种由两层聚丙烯组成的新型假体（称为复合材料），一层是大孔的（称为网片），另一层是透明的（称为薄膜），以评估其在不诱导细胞死亡的情况下促进细胞增殖的能力。同时还检查了炎症过程。
将人成纤维细胞 BJ 接种在多孔板上，其上放置了复合材料或薄膜。在第 7、14 和 21 天，评估细胞生长和活力、胶原蛋白沉积以及白细胞介素 6（IL-6）、白细胞介素 1β（IL-1β）和肿瘤坏死因子-α（TNF-α）的释放。
“体外”方案显示复合材料被人成纤维细胞定植在聚丙烯大孔网片侧，而薄膜上没有细胞生长。在第 14 天至第 21 天观察到的细胞生长减缓伴随着 I 型胶原蛋白沉积增加和明显的成纤维细胞活性增加。炎症细胞因子最初增加，然后从第 14 天开始减少。
由两种不同形态聚丙烯层组成的新型假体可以在面向腹壁的一侧被成纤维细胞定植，而在面向内脏的一侧没有细胞生长。在早期实验中观察到的短暂炎症可能对愈合过程很重要。
