Institute of Molecular Biosciences, Mahidol University, Nakorn Pathom, Thailand.
J Med Virol. 2012 Nov;84(11):1757-70. doi: 10.1002/jmv.23403.
Chikungunya virus (CHIKV) has recently re-emerged causing millions of infections in countries around the Indian Ocean. While CHIKV has a broad host cell range and productively infects a number of different cell types, macrophages have been identified as a potential viral reservoir serving to increase the duration of symptoms. To date no CHIKV interacting protein has been characterized and this study sought to identify CHIKV binding proteins expressed on target cell membranes. Two-dimensional virus overlay identified prohibitin (PHB) as a microglial cell expressed CHIKV binding protein. Co-localization, co-immunoprecipitation as well as antibody and siRNA mediated infection inhibition studies all confirmed a role for PHB in mediating internalization of CHIKV into microglial cells. PHB is the first identified CHIKV receptor protein, and this study is evidence that PHB may play a role in the internalization of multiple viruses.
基孔肯雅病毒（CHIKV）最近再次出现，在印度洋周边国家导致数百万人感染。虽然 CHIKV 具有广泛的宿主细胞范围，并能有效地感染许多不同的细胞类型，但巨噬细胞已被确定为一种潜在的病毒储存库，可延长症状持续时间。迄今为止，尚未鉴定出 CHIKV 相互作用蛋白，本研究旨在鉴定在靶细胞膜上表达的 CHIKV 结合蛋白。二维病毒覆盖鉴定出抑素（PHB）为小胶质细胞表达的 CHIKV 结合蛋白。共定位、共免疫沉淀以及抗体和 siRNA 介导的感染抑制研究均证实 PHB 在介导 CHIKV 进入小胶质细胞内化中起作用。PHB 是第一个鉴定出的 CHIKV 受体蛋白，本研究证明 PHB 可能在多种病毒的内化中起作用。
